The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.
Open accessAbstract
Initiation complexes formed by E. coli ribosomes in the presence of 32P-labeled A protein initiator region from R17 bacteriophage Rna have been treated with colicin E3 and disassembled by exposure to 1% sodium dodecyl sulfate. Electrophoresis on 9% polyacrylamide gels reveals a dissociable complex containing the 30-nucleotide-long messenger fragment and the 50-nucleotide-long colicin fragment, which arises from the 3' terminus of the 16S RNA. The complex is a pure RNA-RNA hybird; it is apparently maintained by a seven-base complementarity between the two RNA fragments. Detection of this mRNA-rRNA complex strongly supports the hypothesis that during the initiation step of protein biosynthesis the 3' end of 16S RNA base pairs with the polypurine stretch common to initiator regions in E. coli and bacteriophage mRNAs. The implications of our findings with respect to the molecular mechanism of initiation site selection and mRNA binding to ribosomes, the role of rRNA in ribosome function, and species specificity in translation are explored.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Dahlberg, A. E., Lund, E., Kjeldgaard, N. O., Bowman, C. M., & Nomura, M. (1973) · Biochemistry · doi:10.1021/bi00729a025 · Cited by 28
Cited by
The Noncoding RNA Revolution—Trashing Old Rules to Forge New Ones
Cech, T. R., & Steitz, J. A. (2014) · Cell · doi:10.1016/j.cell.2014.03.008 · Cited by 2,524
Self-citationInitiation of translation in prokaryotes and eukaryotes
Kozak, M. (1999) · Gene · doi:10.1016/s0378-1119(99)00210-3 · Cited by 1,364
mRNA helicases: the tacticians of translational control
Parsyan, A., Svitkin, Y., Shahbazian, D., Gkogkas, C., Lasko, P., Merrick, W. C., & Sonenberg, N. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3083 · Cited by 323
Weiss, R. B., Dunn, D. M., Dahlberg, A. E., Atkins, J. F., & Gesteland, R. F. (1988) · The EMBO Journal · doi:10.1002/j.1460-2075.1988.tb02969.x · Cited by 230
Spangler, E. A., & Blackburn, E. H. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)88976-1 · Cited by 197
Metadata
- Authors
- Steitz, J. A., & Jakes, K.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1975-12
- Volume
- 72
- Issue
- 12
- Pages
- 4734-4738
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1971308369
- Cited by
- 691
- References
- 2
- FWCI
- 26.88
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli. https://app.cochat.ai/papers/10.1073/pnas.72.12.4734
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- Steitz, J. A., & Jakes, K. (1975). How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.72.12.4734
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