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Clonal growth of bovine vascular endothelial cells: fibroblast growth factor as a survival agent.
Open accessAbstract
Bovine vascular endothelial cells do not grow when cultured at low density unless fibroblast growth factor is included in the culture medium. When endothelial cells obtained from the intimal surface of fetal and adult aortas were seeded at low density (8 cells per cm2), they formed small colonies of large, irregular, vacuolated cells. At very low density (0.3 cells per cm2) they did not survive. The addition of fibroblast growth factor to endothelial cells maintained at such low densities resulted in the formation of vigorously growing colonies of small, uniform cells. Electron microscopy showed that the cultured endothelial cells had the fine structure characteristics of endothelial cells. Immunofluorescence microscopy revealed antihemophilic factor (Factor VIII) antigen in the cells. Our results demonstrated that fibroblast growth factor permits the survival of endothelial cells plated at extremely low cell density. With the use of fibroblast growth factor, endothelial cell clones are easily produced.
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Citations
Cited by
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Matfier, J. P. (1980) · Biology of Reproduction · doi:10.1095/biolreprod23.1.243 · Cited by 396
Gospodarowicz, D., Mescher, A. L., & Birdwell, C. R. (1977) · Experimental Eye Research · doi:10.1016/0014-4835(77)90248-2 · Cited by 337
Self-citationVlodavsky, I., Fielding, P. E., Fielding, C. J., & Gospodarowicz, D. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.1.356 · Cited by 116
Self-citationAntioxydant activity of β-carboline derivatives in the LDL oxidation model
Hadjaz, F., Besret, S., Martin-Nizard, F., Yous, S., Dilly, S., Lebegue, N., Chavatte, P., Duriez, P., Berthelot, P., & Carato, P. (2011) · European Journal of Medicinal Chemistry · doi:10.1016/j.ejmech.2011.03.048 · Cited by 21
Metadata
- Authors
- Gospodarowicz, D., Moran, J., Braun, D., & Birdwell, C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1976-11
- Volume
- 73
- Issue
- 11
- Pages
- 4120-4124
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2086565983
- Cited by
- 414
- References
- 0
- FWCI
- 8.30
- Type
- journal article
- Language
- en
- Open access
- green
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- Gospodarowicz, D., Moran, J., Braun, D., & Birdwell, C. (1976). Clonal growth of bovine vascular endothelial cells: fibroblast growth factor as a survival agent. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.73.11.4120
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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