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Identification of prekallikrein and high-molecular-weight kininogen as a complex in human plasma.
Open accessAbstract
Prekallikrein and high-molecular-weight kininogen were found associated in normal human plasma at a molecular weight of 285,000 as assessed by gel filtration on Sephadex G-200. The molecular weight of prekallikrein in plasma that is deficient in high-molecular-weight kininogen was 115,000. This prekallikrein could be isolated at a molecular weight of 285,000 after plasma deficient in high-molecular-weight kininogen was combined with plasma that is congenitally deficient in prekallikrein. Addition of purified 125I-labeled prekallikrein and high-molecular-weight kininogen to the respective deficient plasma yielded a shift in the molecular weight of prekallikrein, and complex formation could be demonstrated by incubating prekallikrein with high-molecular weight kininogen. This study demonstrates that prekallikrein and high-molecular-weight kininogen are physically associated in plasma as a noncovalently linked complex and may therefore be adsorbed together during surface activation of Hageman factor. The complex is disrupted when these proteins are isolated by ion exchange chromatography.
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Cited by
The Kallikrein-Kinin System: Current and Future Pharmacological Targets
Moreau, M. E., Garbacki, N., Molinaro, G., Brown, N. J., Marceau, F., & Adam, A. (2005) · Journal of Pharmacological Sciences · doi:10.1254/jphs.srj05001x · Cited by 457
Busse, P. J., & Christiansen, S. C. (2020) · New England Journal of Medicine · doi:10.1056/nejmra1808012 · Cited by 445
The kallikrein-kininogen-kinin system: lessons from the quantification of endogenous kinins
Blais, C., Marceau, F., Rouleau, J.-L., & Adam, A. (2000) · Peptides · doi:10.1016/s0196-9781(00)00348-x · Cited by 135
High Molecular Weight Kininogen: A Review of the Structural Literature
Ponczek, M. B. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms222413370 · Cited by 45
Metadata
- Authors
- Mandle, R. J., Colman, R. W., & Kaplan, A. P.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1976-11
- Volume
- 73
- Issue
- 11
- Pages
- 4179-4183
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2092462079
- Cited by
- 350
- References
- 0
- FWCI
- 6.91
- Type
- journal article
- Language
- en
- Open access
- green
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- Mandle, R. J., Colman, R. W., & Kaplan, A. P. (1976). Identification of prekallikrein and high-molecular-weight kininogen as a complex in human plasma. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.73.11.4179
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