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Hydrolytic stability of helical RNA: a selective advantage for the natural 3',5'-bond.
Open accessAbstract
Dodecaadenylic acid containing a single 2', 5'-linkage at a defined position was formed by the coupling of two hexamers on a poly(U) template at 2 degrees. The rate of hydrolysis of this dodecamer was compared with that of a dodecamer that contained only the natural 3', 5'-linkages. At 40 degrees, in 1 M aqueous ethylenediamine at pH 8 in the absence of poly(U), both dodecamers hydrolyzed at comparable rates, but the addition of two equivalents of poly(U) caused a 7-fold increase in the initial rate of hydrolysis of the oligomer containing the 2', 5'-bond, and a 5-fold decrease in the initial rate of hydrolysis of the natural oligomer. When the oligomers are fully constrained in helical form, the ratio of the rates of cleavage of one 2', 5'-bond to one 3', 5'-bond under these conditions is probably about 900:1. The use of the 3', 5'-bond, in combination with a right-handed helix, appears to have had a large selective advantage over the use of the 2', 5'-bond for the storage of genetic information.
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Cited by
Genetic Control by a Metabolite Binding mRNA
Nahvi, A., Sudarsan, N., Ebert, M. S., Zou, X., Brown, K. L., & Breaker, R. R. (2002) · Chemistry & Biology · doi:10.1016/s1074-5521(02)00224-7 · Cited by 801
Chemical etiology of nucleic acid structure
Eschenmoser, A. (1999) · Science · doi:10.1126/science.284.5423.2118 · Cited by 611
Li, Y., & Breaker, R. R. (1999) · Journal of the American Chemical Society · doi:10.1021/ja990592p · Cited by 587
Relationship between internucleotide linkage geometry and the stability of RNA
SOUKUP, G. A., & BREAKER, R. R. (1999) · RNA · doi:10.1017/s1355838299990891 · Cited by 549
Oivanen, M., Kuusela, S., & Lönnberg, H. (1998) · Chemical Reviews · doi:10.1021/cr960425x · Cited by 438
Metadata
- Authors
- Usher, D. A., & McHale, A. H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1976-04
- Volume
- 73
- Issue
- 4
- Pages
- 1149-1153
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2069955207
- Cited by
- 179
- References
- 0
- FWCI
- 1.41
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Hydrolytic stability of helical RNA: a selective advantage for the natural 3',5'-bond. https://app.cochat.ai/papers/10.1073/pnas.73.4.1149
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- Usher, D. A., & McHale, A. H. (1976). Hydrolytic stability of helical RNA: a selective advantage for the natural 3',5'-bond. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.73.4.1149
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