The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Polypeptides with nonsuppressible insulin-like and cell-growth promoting activities in human serum: isolation, chemical characterization, and some biological properties of forms I and II.
Open accessAbstract
Serum contains a polypeptide with insulin-like activity not suppressible by insulin antibodies (NSILA). A large-scale isolation procedure for NSILA is described, starting from an acid ethanol extract of a Cohn fraction (precipitate B) obtained from human plasma. Two homogenous polypeptides with insulin-like and cell-growth promoting activities could be isolated by gel filtration, ion exchange chromatography, and preparative polyacrylamide gel electrophoresis. Both components are slightly basic polypeptides with a minimal molecular weight of 5800 +/- 400. Both are single-chain molecules with two intrachain disulfide bridges each and no free sulfhydryl groups. NSILA I and II differ, however, in their amino acid compositions. The N-terminal amino acid sequences are Gly-Pro-Glu- in NSILA I, and Ala-Tyr-Arg- and Tyr-Arg- in NSILA II. Both NSILA I and II enhance net gas exchange in adipose tissue with a specific activity 60 times lower than that of insulin. In the range of 1-50 ng/ml, both substances stimulate [3H]thymidine incorporation into DNA of chick embryo fibroblasts. The same effect can be obtained with insulin but only at concentrations 50-100 times higher than those of NSILA. These results suggest that NSILA I and II are two forms of an insulin-like hormone with predominating effects on cell and tissue growth parameters.
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Metadata
- Authors
- Rinderknecht, E., & Humbel, R. E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1976-07
- Volume
- 73
- Issue
- 7
- Pages
- 2365-2369
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2053134566
- Cited by
- 324
- References
- 13
- FWCI
- 17.61
- Type
- journal article
- Language
- en
- Open access
- green
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- Rinderknecht, E., & Humbel, R. E. (1976). Polypeptides with nonsuppressible insulin-like and cell-growth promoting activities in human serum: isolation, chemical characterization, and some biological properties of forms I and II. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.73.7.2365
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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