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Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.
Open accessAbstract
We present a simple method for directly correlating structural gene sequences in DNA with their corresponding mRNAs. This is based upon the fact that mRNA hybridized with its complementary DNA will not direct the cell-free synthesis of a complete polypeptide. Full translational activity of the mRNA is recovered upon the heat melting of the hybrid. Utilizing the rabbit beta globin clone PbetaG1, we demonstrate the application of hybrid-arrested translation for the identification of structural gene sequences within recombinant DNA molecules. In addition, the method is used to locate and order precisely several adenovirus 2 polypeptides within the viral genome.
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Cited by
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Opalinska, J. B., & Gewirtz, A. M. (2002) · Nature Reviews Drug Discovery · doi:10.1038/nrd837 · Cited by 537
Nucleotide sequence of cauliflower mosaic virus DNA
Franck, A., Guilley, H., Jonard, G., Richards, K., & Hirth, L. (1980) · Cell · doi:10.1016/0092-8674(80)90136-1 · Cited by 477
siRNA-based approaches in cancer therapy
Devi, G. R. (2006) · Cancer Gene Therapy · doi:10.1038/sj.cgt.7700931 · Cited by 353
Filter replicas and permanent collections of recombinant DNA plasmids
Gergen, J. P., Stern, R. H., & Wensink, P. C. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.8.2115 · Cited by 353
Metadata
- Authors
- Paterson, B. M., Roberts, B. E., & Kuff, E. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1977-10
- Volume
- 74
- Issue
- 10
- Pages
- 4370-4374
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2005333148
- Cited by
- 536
- References
- 0
- FWCI
- 16.93
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation. https://app.cochat.ai/papers/10.1073/pnas.74.10.4370
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- Paterson, B. M., Roberts, B. E., & Kuff, E. L. (1977). Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.74.10.4370
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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