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Cited by 353

The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.

Filter replicas and permanent collections of recombinant DNA plasmids

Open access
Gergen, J.Peter, Stern, Ralph H., Wensink, Pieter C.·1979·Nucleic Acids Research

Abstract

A permanent, ordered collection of 23,000 recombinant DNA plasmids containing Drosophila melanogaster DNA has been established. Simple and practical methods for storing and manipulating this collection were developed. In addition, an improved, simple and inexpensive method for making paper filter replicas of such an ordered collection and of a high density (10,000 colonies/petri dish) unordered collection was developed. These filter replicas are suitable for nucleic acid hybridization screens of recombinant DNA colinies and each filter replica can be used for many (greater than 5) successive screens. The kinetics of this hybridization reaction were examined and allow design of experiments that detect colony complementarity to a nucleic acid that is 0.5% of the hybridization probe.

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Crossref · 10.1093/nar/7.8.2115

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Metadata

Authors
Gergen, J. P., Stern, R. H., & Wensink, P. C.
Venue
Nucleic Acids Research
Published
1979
Volume
7
Issue
8
Pages
2115-2136
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
OpenAlex
W2059536036
Cited by
353
References
26
FWCI
8.51
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Filter replicas and permanent collections of recombinant DNA plasmids. https://app.cochat.ai/papers/10.1093/nar/7.8.2115
The paper
Gergen, J. P., Stern, R. H., & Wensink, P. C. (1979). Filter replicas and permanent collections of recombinant DNA plasmids. Nucleic Acids Research. https://doi.org/10.1093/nar/7.8.2115

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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