The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Filter replicas and permanent collections of recombinant DNA plasmids
Open accessAbstract
A permanent, ordered collection of 23,000 recombinant DNA plasmids containing Drosophila melanogaster DNA has been established. Simple and practical methods for storing and manipulating this collection were developed. In addition, an improved, simple and inexpensive method for making paper filter replicas of such an ordered collection and of a high density (10,000 colonies/petri dish) unordered collection was developed. These filter replicas are suitable for nucleic acid hybridization screens of recombinant DNA colinies and each filter replica can be used for many (greater than 5) successive screens. The kinetics of this hybridization reaction were examined and allow design of experiments that detect colony complementarity to a nucleic acid that is 0.5% of the hybridization probe.
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Cites
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Isolation and nucleotide sequence of a cDNA encoding the precursor of mouse nerve growth factor
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Metadata
- Authors
- Gergen, J. P., Stern, R. H., & Wensink, P. C.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1979
- Volume
- 7
- Issue
- 8
- Pages
- 2115-2136
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2059536036
- Cited by
- 353
- References
- 26
- FWCI
- 8.51
- Type
- journal article
- Language
- en
- Open access
- green
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- Gergen, J. P., Stern, R. H., & Wensink, P. C. (1979). Filter replicas and permanent collections of recombinant DNA plasmids. Nucleic Acids Research. https://doi.org/10.1093/nar/7.8.2115
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