The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Human leukocytic pyrogen: purification and development of a radioimmunoassay.
Open accessAbstract
Leukocytic pyrogen is a small endogenous protein that mediates fever. Because of the limitations of bioassays, circulating leukocytic pyrogen has not been demonstrated during fever in humans. The pyrogen was produced in vitro after phagocytosis of staphylococci by blood monocytes. Antibody against the pyrogen was obtained from rabbits immunized with leukocytic pyrogen and the antiserum was purified by solid-phase immunoadsorbants. Purified antibody to the pyrogen was attached to activated Sepharose 4B and used in conjunction with gel filtration to purify the pyrogen. The pyrogen was labeled with 125I and further purified by gel filtration and ion-exchange chromatography. The final preparation of 125I-labeled pyrogen demonstrated a homogeneous band during isoelectric focusing and other separation procedures. With antibody to pyrogen attached to Sepharose, less than 0.1 of a rabbit pyrogenic dose of human leukocytic pyrogen inhibited the binding of 125I-labeled pyrogen to this immunoadsorbant, and this inhibition was not affected by the presence of human serum. Thus, a radioimmunoassay for human leukocytic pyrogen has been developed that may be used to detect circulating pyrogen during fever in humans.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Bolton, A. E., & Hunter, W. M. (1973) · Biochemical Journal · doi:10.1042/bj1330529 · Cited by 3,254
Selective enzyme purification by affinity chromatography.
Cuatrecasas, P., Wilchek, M., & Anfinsen, C. B. (1968) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.61.2.636 · Cited by 1,223
DEMONSTRATION AND CHARACTERIZATION OF TWO DISTINCT HUMAN LEUKOCYTIC PYROGENS
Dinarello, C. A., Goldin, N. P., & Wolff, S. M. (1974) · The Journal of Experimental Medicine · doi:10.1084/jem.139.6.1369 · Cited by 229
Self-citationAtkins, E., & Bodel, P. T. (1972) · New England Journal of Medicine · doi:10.1056/nejm197201062860109 · Cited by 104
Human Leukocyte Pyrogen Producing Fever in Rabbits.
Bodel, P. T., & Atkins, E. (1966) · Experimental Biology and Medicine · doi:10.3181/00379727-121-30931 · Cited by 53
Cited by
Overview of the IL ‐1 family in innate inflammation and acquired immunity
Dinarello, C. A. (2018) · Immunological Reviews · doi:10.1111/imr.12621 · Cited by 1,866
Self-citationDinarello, C. A. (1988) · The FASEB Journal · doi:10.1096/fasebj.2.2.3277884 · Cited by 1,532
Self-citationImmunoregulatory Feedback Between Interleukin-1 and Glucocorticoid Hormones
Besedovsky, H., del Rey, A., Sorkin, E., & Dinarello, C. A. (1986) · Science · doi:10.1126/science.3014662 · Cited by 1,519
Self-citationTumor necrosis factor (cachectin) is an endogenous pyrogen and induces production of interleukin 1.
Dinarello, C. A., Cannon, J. G., Wolff, S. M., Bernheim, H. A., Beutler, B., Cerami, A., Figari, I. S., Palladino, M. A., & O'Connor, J. V. (1986) · The Journal of experimental medicine · doi:10.1084/jem.163.6.1433 · Cited by 1,472
Self-citationNucleotide sequence of human monocyte interleukin 1 precursor cDNA.
Auron, P. E., Webb, A. C., Rosenwasser, L. J., Mucci, S. F., Rich, A., Wolff, S. M., & Dinarello, C. A. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.24.7907 · Cited by 1,042
Self-citation
Metadata
- Authors
- Dinarello, C. A., Renfer, L., & Wolff, S. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1977-10
- Volume
- 74
- Issue
- 10
- Pages
- 4624-4627
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2009545343
- Cited by
- 272
- References
- 5
- FWCI
- 7.81
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Human leukocytic pyrogen: purification and development of a radioimmunoassay. https://app.cochat.ai/papers/10.1073/pnas.74.10.4624
- The paper
- Dinarello, C. A., Renfer, L., & Wolff, S. M. (1977). Human leukocytic pyrogen: purification and development of a radioimmunoassay. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.74.10.4624
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
