The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Direction of active sliding of microtubules in Tetrahymena cilia.
Open accessAbstract
Axonemes of protozoan (Tetrahymena thermophila BIII) cilia, isolated by the dibucaine method, were treated briefly with trypsin after removal of the ciliary membranes by treatment with Triton X-100. After attachment to polylysine-coated surfaces, the partially digested axonemes remained mainly intact cylinders. Such attached axonemes can be treated with ATP, which induces microtubles sliding. ATP-treated preparations showed disrupted axonemes in which doublets had telescoped out of the original cylinders. These could be captured in place for electron microscopy after critical point drying. Images of this type were used to determine relative movement between adjacent doublet microtubules. Each doublet actively slid relative to its neighbors in a single direction, in which the polarity of force generation of the dynein arms was from base to tip.
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Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
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Adhesion of cells to surfaces coated with polylysine. Applications to electron microscopy.
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Self-citationAdenosine Triphosphate-Induced Sliding of Tubules in Trypsin-Treated Flagella of Sea-Urchin Sperm
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Self-citationTHE STRUCTURAL BASIS OF CILIARY BEND FORMATION
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Self-citationStructural polarity and directional growth of microtubules of Chlamydomonas flagella
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EFFECTS OF TRYPSIN DIGESTION ON FLAGELLAR STRUCTURES AND THEIR RELATIONSHIP TO MOTILITY
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Membrane renewal after dibucaine deciliation of Tetrahymena
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Cited by
Cytoplasmic Dynein Heavy Chain 1b Is Required for Flagellar Assembly in Chlamydomonas
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Cytoplasmic dynein is a minus end-directed motor for membranous organelles
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Subfractionation of Chlamydomonas 18 S dynein into two unique subunits containing ATPase activity.
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Dougherty, G. W., Mizuno, K., Nöthe-Menchen, T., Ikawa, Y., Boldt, K., Ta-Shma, A., Aprea, I., Minegishi, K., Pang, Y.-P., Pennekamp, P., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-19113-0 · Cited by 88
Metadata
- Authors
- Sale, W. S., & Satir, P.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1977-05
- Volume
- 74
- Issue
- 5
- Pages
- 2045-2049
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2079566817
- Cited by
- 320
- References
- 12
- FWCI
- 7.20
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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