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Cited by 915

The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.

Different intermediate-sized filaments distinguished by immunofluorescence microscopy.

Open access
Franke, W W, Schmid, E, Osborn, M, Weber, K·1978·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.75.10.5034
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Abstract

The major protein of intermediate-sized filaments in mouse 3T3 cells, for which the name vimentin is proposed, has a molecular weight of 57,000. Antibodies against vimentin and antibodies against prekeratin have been used in parallel in immunofluorescence microscopy on a variety of cultured cells as well as on frozen tissue sections. Both antibodies decorate extended wavy arrays of filaments that are different from microfilaments and microtubules. Intermediate filament bundles decorated by antibodies against prekeratin are predominant in many epithelial cells, including epithelia-derived tumor cells, and are not decorated by antibodies to vimentin. In contrast, intermediate filaments decorated by antibodies against vimentin are widespread among nonmuscle cells of mesenchymal origin, including transformed cells, and also occur in other cells. Perinuclear whorls of aggregates of intermediate filaments induced by prolonged treatment with Colcemid generally show strong decoration with antibodies against vimentin. No significant reaction with either antiserum has been observed in muscle structures or in brain nerve tissue. These observations show that intermediate filaments with similar ultrastructure and solubility characteristics can be distinguished immunologically.

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Metadata

Authors
Franke, W. W., Schmid, E., Osborn, M., & Weber, K.
Venue
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Published
1978-10
Volume
75
Issue
10
Pages
5034-5038
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.75.10.5034
OpenAlex
W1995789984
Cited by
915
References
12
FWCI
25.60
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Different intermediate-sized filaments distinguished by immunofluorescence microscopy. https://app.cochat.ai/papers/10.1073/pnas.75.10.5034
The paper
Franke, W. W., Schmid, E., Osborn, M., & Weber, K. (1978). Different intermediate-sized filaments distinguished by immunofluorescence microscopy. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.75.10.5034

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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