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Transformation of yeast.
Open accessAbstract
A stable leu2- yeast strain has been transformed to LEU2+ by using a chimeric ColE1 plasmid carrying the yeast leu2 gene. We have used recently developed hybridization and restriction endonuclease mapping techniques to demonstrate directly the presence of the transforming DNA in the yeast genome and also to determine the arrangement of the sequences that were introduced. These studies show that ColE1 DNA together with the yeast sequences can integrate into the yeast chromosomes. This integration may be additive or substitutive. The bacterial plasmid sequences, once integrated, behave as a simple Mendelian element. In addition, we have determined the genetic linkage relationships for each newly introduced LEU2+ allele with the original leu2- allele. These studies show that the transforming squences integrate not only in the leu2 region but also in several other chromosomal locations.
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Citations
Cited by
Baker Brachmann, C., Davies, A., Cost, G. J., Caputo, E., Li, J., Hieter, P., & Boeke, J. D. (1998) · Yeast · doi:10.1002/(sici)1097-0061(19980130)14:2<115::aid-yea204>3.0.co;2-2 · Cited by 3,401
The double-strand-break repair model for recombination
Szostak, J. W., Orr-Weaver, T. L., Rothstein, R. J., & Stahl, F. W. (1983) · Cell · doi:10.1016/0092-8674(83)90331-8 · Cited by 2,437
Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells
Thomas, K. R., & Capecchi, M. R. (1987) · Cell · doi:10.1016/0092-8674(87)90646-5 · Cited by 2,355
High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
Struhl, K., Stinchcomb, D. T., Scherer, S., & Davis, R. W. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.3.1035 · Cited by 1,456
Yeast transformation: a model system for the study of recombination.
Orr-Weaver, T. L., Szostak, J. W., & Rothstein, R. J. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.10.6354 · Cited by 1,455
Metadata
- Authors
- Hinnen, A., Hicks, J. B., & Fink, G. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1978-04
- Volume
- 75
- Issue
- 4
- Pages
- 1929-1933
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2022016707
- Cited by
- 2,205
- References
- 0
- FWCI
- 28.85
- Type
- journal article
- Language
- en
- Open access
- green
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- Hinnen, A., Hicks, J. B., & Fink, G. R. (1978). Transformation of yeast. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.75.4.1929
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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