The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Yeast transformation: a model system for the study of recombination.
Open accessAbstract
DNA molecules that integrate into yeast chromosomes during yeast transformation do so by homologous recombination. We have studied the way in which circular and linear molecules recombine with homologous chromosomal sequences. We show that DNA ends are highly recombinogenic and interact directly with homologous sequences. Circular hybrid plasmids can integrate by a single reciprocal crossover, but only at a low frequency. Restriction enzyme digestion within a region homologous to yeast chromosomal DNA greatly enhances the efficiency of integration. Furthermore, if two restriction cuts are made within the same homologous sequence, thereby removing an internal segment of DNA, the resulting deleted-linear molecules are still able to transform at a high frequency. Surprisingly, the integration of these gapped-linear molecules results in replacement of the missing segment using chromosomal information. The final structure is identical to that obtained from integration of a circular molecule. The integration of linear and gapped-linear molecules, but not of circular molecules, is blocked by the rad52-1 mutation. Consideration of models for plasmid integration and gene conversion suggests that RAD52 may be involved in the DNA repair synthesis required for these processes. Implications of this work for the isolation of integrative transformants, fine-structure mapping, and the cloning of mutations are discussed.
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Cites
Detection of specific sequences among DNA fragments separated by gel electrophoresis
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A general model for genetic recombination.
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[49] Rapid DNA isolations for enzymatic and hybridization analysis
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The repair of double-strand breaks in DNA: A model involving recombination
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Cited by
The double-strand-break repair model for recombination
Szostak, J. W., Orr-Weaver, T. L., Rothstein, R. J., & Stahl, F. W. (1983) · Cell · doi:10.1016/0092-8674(83)90331-8 · Cited by 2,437
Self-citationSite-directed mutagenesis by gene targeting in mouse embryo-derived stem cells
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Telomere length predicts replicative capacity of human fibroblasts.
Allsopp, R. C., Vaziri, H., Patterson, C., Goldstein, S., Younglai, E. V., Futcher, A. B., Greider, C. W., & Harley, C. B. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.21.10114 · Cited by 2,200
Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae
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Yeast/E. coli shuttle vectors with multiple unique restriction sites
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Metadata
- Authors
- Orr-Weaver, T. L., Szostak, J. W., & Rothstein, R. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1981-10
- Volume
- 78
- Issue
- 10
- Pages
- 6354-6358
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1985910275
- Cited by
- 1,455
- References
- 19
- FWCI
- 21.49
- Type
- journal article
- Language
- en
- Open access
- green
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- Orr-Weaver, T. L., Szostak, J. W., & Rothstein, R. J. (1981). Yeast transformation: a model system for the study of recombination. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.78.10.6354
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
