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Multiple phosphorylation sites in protein I and their differential regulation by cyclic AMP and calcium.
Open accessAbstract
The phosphorylation of protein I, a specific neuronal protein, has been found to be regulated both by cyclic AMP (cAMP) and by calcium, in intact as well as in lysed synaptosome preparations from rat brain. In order to determine the phosphorylation site(s) of protein I that were regulated by cAMP and calcium, protein I was purified after it was phosphorylated under various conditions. This purified protein I was then subjected either to peptide mapping after limited proteolysis in sodium dodecyl sulfate/polyacrylamide gels or to tryptic fingerprinting. 8-Br-cAMP selectively increased the phosphorylation of the same protein I peptide fragment in both intact and lysed synaptosomes. Depolarization-induced calcium influx into intact synaptosomes, or the addition of calcium to lysed synaptosomes, caused a stimulation of the phosphorylation not only of this peptide but also of other distinct peptides. Differential regulation by cAMP and calcium of the phosphorylation of multiple sites on the same neuronal protein may provide a molecular basis for interactions between these two second-messenger systems in certain nerve terminal functions.
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Cited by
Synaptic Vesicle Phosphoproteins and Regulation of Synaptic Function
Greengard, P., Valtorta, F., Czernik, A. J., & Benfenati, F. (1993) · Science · doi:10.1126/science.8430330 · Cited by 1,312
Self-citationThe role of protein phosphorylation in neural and hormonal control of cellular activity
Cohen, P. (1982) · Nature · doi:10.1038/296613a0 · Cited by 1,123
Miller, S. (1986) · Cell · doi:10.1016/0092-8674(86)90008-5 · Cited by 864
The synapsins: Key actors of synapse function and plasticity
Cesca, F., Baldelli, P., Valtorta, F., & Benfenati, F. (2010) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2010.04.006 · Cited by 654
Llinás, R., McGuinness, T. L., Leonard, C. S., Sugimori, M., & Greengard, P. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.9.3035 · Cited by 640
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Metadata
- Authors
- Huttner, W. B., & Greengard, P.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1979-10
- Volume
- 76
- Issue
- 10
- Pages
- 5402-5406
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2032862924
- Cited by
- 280
- References
- 0
- FWCI
- 9.02
- Type
- journal article
- Language
- en
- Open access
- green
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- Huttner, W. B., & Greengard, P. (1979). Multiple phosphorylation sites in protein I and their differential regulation by cyclic AMP and calcium. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.76.10.5402
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