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Diglyceride lipase: a pathway for arachidonate release from human platelets.
Open accessAbstract
We provide evidence that the mechanism for arachidonate release from stimulated human platelets involves two enzymes: a phosphatidylinositol-specific phospholipase C (EC 3.1.4.10) and a diglyceride lipase. After incubation of platelets with thrombin for 15 seconds, 1.2 nmol of 1-stearoyl-2-arachidonoyl diglyceride per 10(9) platelets, was isolated. Arachidonate was released from this substrate by the action of diglyceride lipase located in the particulate fraction of platelets. The enzyme has a pH optimum of 7.0, is stimulated by calcium ions and reduced glutathione, and liberates 31 nmol of fatty acid per min per mg of platelet particulate protein. The diglyceride lipase has sufficient activity to account for the 5-10 nmol of arachidonate released per 10(9) platelets upon thrombin stimulation. That only arachidonate is released upon thrombin stimulation may be explained by the fact that the diglyceride substrate in platelets contains only arachidonate in the 2 position. The lipase activity found in platelet membranes can also hydrolyze the 1-position fatty acid. Stearate is not released when intact platelets are stimulated with thrombin, and the fate of this fatty acid remains to be elucidated.
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Cited by
Castagna, M., Takai, Y., Kaibuchi, K., Sano, K., Kikkawa, U., & Nishizuka, Y. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)34459-4 · Cited by 4,725
Multiple Functions of Endocannabinoid Signaling in the Brain
Katona, I., & Freund, T. F. (2012) · Annual Review of Neuroscience · doi:10.1146/annurev-neuro-062111-150420 · Cited by 630
Wolfe, L. S. (1982) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1982.tb10847.x · Cited by 573
Release and metabolism of arachidonic acid in human neutrophils.
Walsh, C. E., Waite, B. M., Thomas, M. J., & DeChatelet, L. R. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)68951-9 · Cited by 279
More surprises lying ahead. The endocannabinoids keep us guessing
Piomelli, D. (2014) · Neuropharmacology · doi:10.1016/j.neuropharm.2013.07.026 · Cited by 128
Metadata
- Authors
- Bell, R. L., Kennerly, D. A., Stanford, N., & Majerus, P. W.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1979-07
- Volume
- 76
- Issue
- 7
- Pages
- 3238-3241
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1984787065
- Cited by
- 687
- References
- 0
- FWCI
- 22.28
- Type
- journal article
- Language
- en
- Open access
- green
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- Bell, R. L., Kennerly, D. A., Stanford, N., & Majerus, P. W. (1979). Diglyceride lipase: a pathway for arachidonate release from human platelets. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.76.7.3238
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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