The DOI matches its Crossref record.
Growth of Madin-Darby canine kidney epithelial cell (MDCK) line in hormone-supplemented, serum-free medium.
Open accessAbstract
Madin-Darby canine kidney (MDCK) cells can grow in synthetic medium supplemented with five factors--insulin, transferrin, prostaglandin E1, hydrocortisone, and triiodothyronine--as a serum substitute. These five factors permit growth for 1 month in the absence of serum and a growth rate equivalent to that observed in serum-supplemented medium. Dibutyryl adenosine 3',5'-cyclic monophosphate substitutes for prostaglandin E1 in the medium. Potential applications of the serum-free medium are discussed. The medium permits a defined analysis of the mechanisms regulating hemicyst formation by hormones and permits the growth of primary kidney epithelial cell cultures in the absence of fibroblast overgrowth.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Serum-free cell culture: a unifying approach
Barnes, D., & Sato, G. (1980) · Cell · doi:10.1016/0092-8674(80)90540-1 · Cited by 959
Self-citationApolipoprotein-mediated transport of nanoparticle-bound drugs across the blood-brain barrier.
Kreuter, J., Shamenkov, D., Petrov, V., Ramge, P., Cychutek, K., Koch-Brandt, C., & Alyautdin, R. (2002) · Journal of drug targeting · doi:10.1080/10611860290031877 · Cited by 801
Cholesterol Is Required for Surface Transport of Influenza Virus Hemagglutinin
Keller, P., & Simons, K. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.140.6.1357 · Cited by 554
Alternatives to the use of fetal bovine serum: Serum-free cell culture
Gstraunthaler, G. (2003) · ALTEX · doi:10.14573/altex.2003.4.257 · Cited by 401
Uchida, S., Kwon, H. M., Yamauchi, A., Preston, A. S., Marumo, F., & Handler, J. S. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.17.8230 · Cited by 345
Metadata
- Authors
- Taub, M., Chuman, L., Saier, M. H., & Sato, G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1979-07
- Volume
- 76
- Issue
- 7
- Pages
- 3338-3342
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2073294284
- Cited by
- 304
- References
- 0
- FWCI
- 9.19
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Growth of Madin-Darby canine kidney epithelial cell (MDCK) line in hormone-supplemented, serum-free medium. https://app.cochat.ai/papers/10.1073/pnas.76.7.3338
- The paper
- Taub, M., Chuman, L., Saier, M. H., & Sato, G. (1979). Growth of Madin-Darby canine kidney epithelial cell (MDCK) line in hormone-supplemented, serum-free medium. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.76.7.3338
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
