The DOI matches its Crossref record; 78 of the 78 references it cites were found in a registry or an index.
Cholesterol Is Required for Surface Transport of Influenza Virus Hemagglutinin
Open accessAbstract
Transport from the TGN to the basolateral surface involves a rab/N-ethylmaleimide–sensitive fusion protein (NSF)/soluble NSF attachment protein (SNAP)/SNAP receptor (SNARE) mechanism. Apical transport instead is thought to be mediated by detergent-insoluble sphingolipid–cholesterol rafts. By reducing the cholesterol level of living cells by 60–70% with lovastatin and methyl-β-cyclodextrin, we show that the TGN-to-surface transport of the apical marker protein influenza virus hemagglutinin was slowed down, whereas the transport of the basolateral marker vesicular stomatitis virus glycoprotein as well as the ER-to-Golgi transport of both membrane proteins was not affected. Reduction of transport of hemagglutinin was accompanied by increased solubility in the detergent Triton X-100 and by significant missorting of hemagglutinin to the basolateral membrane. In addition, depletion of cellular cholesterol by lovastatin and methyl-β-cyclodextrin led to missorting of the apical secretory glycoprotein gp-80, suggesting that gp-80 uses a raft-dependent mechanism for apical sorting. Our data provide for the first time direct evidence for the functional significance of cholesterol in the sorting of apical membrane proteins as well as of apically secreted glycoproteins.
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Cites
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Functional rafts in cell membranes
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Self-citationSrimaroeng, C., Cecile, J. P., Walden, R., & Pritchard, J. B. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350077 · Cited by 2,989
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Cholesterol and the cell membrane
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Hibino, H., Inanobe, A., Furutani, K., Murakami, S., Findlay, I., & Kurachi, Y. (2010) · Physiological Reviews · doi:10.1152/physrev.00021.2009 · Cited by 1,539
Cholesterol interactions with phospholipids in membranes
Ohvo-Rekilä, H. (2002) · Progress in Lipid Research · doi:10.1016/s0163-7827(01)00020-0 · Cited by 1,003
Sphingolipid–Cholesterol Rafts Diffuse as Small Entities in the Plasma Membrane of Mammalian Cells
Pralle, A., Keller, P., Florin, E.-L., Simons, K., & Hörber, J. K. H. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.148.5.997 · Cited by 987
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Metadata
- Authors
- Keller, P., & Simons, K.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1998-03-23
- Volume
- 140
- Issue
- 6
- Pages
- 1357-1367
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2138059603
- Cited by
- 554
- References
- 78
- FWCI
- 15.91
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
