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Detection of substance P in the central nervous system by a monoclonal antibody.
Open accessAbstract
Peptides with transmitter-like characteristics are being found in many brain areas. The application of immunocytochemical and radioimmunoassay methods has contributed much to the clarification of these neuronal systems. Here we report the development of a rat monoclonal antibody produced by a hybrid myeloma and its application to the study of one of these peptides, substance P. The hybrid clone, isolated after fusion of mouse myeloma cells with hyperimmune rat spleen cells, allowed us to obtain a standardized and permanent source of monoclonal substance P antibodies in a culture cell system. This antibody recognizes the COOH-terminal part of substance P in radioimmunoassay down to 10-20 fmol. It does not crossreact with other known mammalian brain peptides tested. By immunofluorescence the antibody was shown to bind specifically and with a remarkably low background to nerve terminals and cell bodies located in clearly defined nuclear organizations of the central nervous system.
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Cited by
Todd, A. J., Hughes, D. I., Polgár, E., Nagy, G. G., Mackie, M., Ottersen, O. P., & Maxwell, D. J. (2003) · European Journal of Neuroscience · doi:10.1046/j.1460-9568.2003.02406.x · Cited by 435
Gibbins, I. L., Furness, J. B., & Costa, M. (1987) · Cell and Tissue Research · doi:10.1007/bf00218210 · Cited by 351
DeFelipe, J., Hendry, S. H. C., Hashikawa, T., Molinari, M., & Jones, E. G. (1990) · Neuroscience · doi:10.1016/0306-4522(90)90097-n · Cited by 231
Voorn, P., & Buijs, R. M. (1983) · Brain Research · doi:10.1016/0006-8993(83)90809-0 · Cited by 112
Pagliardini, S., Ren, J., Wevrick, R., & Greer, J. J. (2005) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)62964-1 · Cited by 99
Metadata
- Authors
- Cuello, A. C., Galfre, G., & Milstein, C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1979-07
- Volume
- 76
- Issue
- 7
- Pages
- 3532-3536
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1967770688
- Cited by
- 591
- References
- 0
- FWCI
- 8.88
- Type
- journal article
- Language
- en
- Open access
- green
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- Cuello, A. C., Galfre, G., & Milstein, C. (1979). Detection of substance P in the central nervous system by a monoclonal antibody. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.76.7.3532
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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