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Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
Open accessAbstract
A broad host range cloning vehicle that can be mobilized at high frequency into Gram-negative bacteria has been constructed from the naturally occurring antibiotic resistance plasmid RK2. The vehicle is 20 kilobase pairs in size, encodes tetracycline resistance, and contains two single restriction enzyme sites suitable for cloning. Mobilization is effected by a helper plasmid consisting of the RK2 transfer genes linked to a ColE1 replicon. By use of this plasmid vehicle, a gene bank of the DNA from a wild-type strain of Rhizobium meliloti has been constructed and established in Escherichia coli. One of the hybrid plasmids in the bank contains a DNA insert of approximately 26 kilobase pairs which has homology to the nitrogenase structural gene region of Klebsiella pneumoniae.
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Cited by
Wolk, C. P., Vonshak, A., Kehoe, P., & Elhai, J. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.5.1561 · Cited by 328
Wallaart, T. E., Bouwmeester, H. J., Hille, J., Poppinga, L., & Maijers, N. C. A. (2001) · Planta · doi:10.1007/s004250000428 · Cited by 242
Romano, C. P., Hein, M. B., & Klee, H. J. (1991) · Genes & Development · doi:10.1101/gad.5.3.438 · Cited by 232
Taton, A., Lis, E., Adin, D. M., Dong, G., Cookson, S., Kay, S. A., Golden, S. S., & Golden, J. W. (2012) · PLoS ONE · doi:10.1371/journal.pone.0030901 · Cited by 70
A Widespread Deferoxamine‐Mediated Iron‐Uptake System in Vibrio vulnificus
Kim, C., Park, Y., & Shin, S. (2007) · The Journal of Infectious Diseases · doi:10.1086/523108 · Cited by 49
Metadata
- Authors
- Ditta, G., Stanfield, S., Corbin, D., & Helinski, D. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1980-12
- Volume
- 77
- Issue
- 12
- Pages
- 7347-7351
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1987355768
- Cited by
- 2,667
- References
- 0
- FWCI
- 23.05
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. https://app.cochat.ai/papers/10.1073/pnas.77.12.7347
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- Ditta, G., Stanfield, S., Corbin, D., & Helinski, D. R. (1980). Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.77.12.7347
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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