Inactivation of auxin in tobacco transformed with the indoleacetic acid-lysine synthetase gene of Pseudomonas savastanoi.
Open accessAbstract
The iaaL gene of Pseudomonas syringae, subspecies savastanoi, encodes an indoleacetic acid (IAA)-lysine synthetase. To determine the effects of converting IAA to IAA-lysine in whole plants, the iaaL gene was fused to a constitutive plant promoter and introduced into tobacco plants. Biochemical analyses show that endogenous IAA is reduced by up to 19-fold in iaaL plants. Tobacco plants expressing the iaaL gene display reduced apical dominance, reduced rooting, and inhibition of vascular differentiation. The phenotypic effects of iaaL gene expression are reverted by crossing iaaL plants with plants that overproduce IAA. These data indicate that iaaL can act as an anti-auxin gene in vivo and confirm the role of auxin in the control of apical dominance, root growth, and vascular differentiation in whole plants.
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Auxin-resistant mutants of Arabidopsis thaliana with an altered morphology
Estelle, M. A., & Somerville, C. (1987) · Molecular and General Genetics MGG · doi:10.1007/bf00333575 · Cited by 576 · see citing works
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Research works printed but not linked: 12
- Bandurski, R.S. 1984. Metabolism of indole-3-acetic acid. In The biosynthesis and metabolism of plant hormones (ed. A. Cro- zier and J.R. Hillman), pp. 183-200. Cambridge University Press, Cambridge.
- Cohen, J.D. and K. Bialek. 1984. The biosynthesis of indole- 3-acetic acid in higher plants. In The biosynthesis and me- tabolism of plant hormones {ed. A. Crozier and J.R. Hill- man), pp. 165-181. Cambridge University Press, Cambridge.
- Ditta, G., S. Stanfield, D. Corbin, and D. Helinski. 1980. Broad host range DNA cloning system for gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci. 77: 7347-7351.
- Hagen, G. 1987. The control of gene expression by auxin. In Plant hormones and their role in plant growth and develop- ment (ed. P.J. Davies), pp. 149-163. Martinus Nijhoff, Boston.
- Hutzinger, O. and T. Kosuge. 1968a. 3-indole-acetyl-e-L-lysine, a new conjugate of 3-indoleacetic acid produced by Pseudomonas savastanoi. In Biochemistry and physiology of plant growth substances (ed. F. Wightman and G. Setter- field), pp. 183-194.
- Kaufman, P.B. and I. Song. 1987. Hormones and the orientation of growth. In Plant hormones and their role in plant growth and development (ed. P.J. Davies), pp. 375-392.
- Kunkel, T.A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. 82: 488-492.
- Napoli, C., C. Lemieux, and R. Jorgensen. 1990. Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of the homologous genes in trans. Hant Cell 2: 279-289.
- Roberto, F.F., H.J. Klee, F. White, R. Nordeen, and T. Kosuge. 1990. Expression and fine structure of the gene encoding N-e-indole-3-acetyl-L-lysine synthetase from Pseudomonas savastanoi. Proc. Natl. Acad. Sci. 87: 5797-5801.
- Schneider, E.A. and F. Wightman. 1978. Auxins. In Phytohor- mones and related compounds--A comprehensive treatise, (ed. D.D. Letham, P.B. Goodwin, and T.J. Higgins), vol. 1, pp. 29-105. Elsevier/North Holland Biomedical Press, New York.
- Tamas, I.A. 1987. Hormonal regulation of apical dominance. In Plant hormones and their roles in plant growth and devel- opment (ed. P.J. Davies), pp. 349--410.
- Thimann, K.V. and F. Skoog. 1934. On the inhibition of bud development and other functions of growth substance in Vi- cia faba. Proc. R. Soc. Bl14: 317-339.
Cited by
Sorted by citations
Cline, M. G. (1991) · The Botanical Review · doi:10.1007/bf02858771 · Cited by 380 · see citing works
The hormonal regulation of axillary bud growth in <i>Arabidopsis</i>
Chatfield, S. P., Stirnberg, P., Forde, B. G., & Leyser, O. (2000) · The Plant Journal · doi:10.1046/j.1365-313x.2000.00862.x · Cited by 284 · see citing works
Beveridge, C. A., Symons, G. M., & Turnbull, C. G. N. (2000) · Plant Physiology · doi:10.1104/pp.123.2.689 · Cited by 162 · see citing works
<i>AXR1</i> Acts after Lateral Bud Formation to Inhibit Lateral Bud Growth in Arabidopsis
Stirnberg, P., Chatfield, S. P., & Leyser, H. M. O. (1999) · Plant Physiology · doi:10.1104/pp.121.3.839 · Cited by 152 · see citing works
- Authors
- Romano, C. P., Hein, M. B., & Klee, H. J.
- Affiliations
- Monsanto (United States) (US)
- ORCID
- Klee, H J
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1991-03
- Volume
- 5
- Issue
- 3
- Pages
- 438-446
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2057439433
- Cited by
- 232
- References
- 1
- Citations by year
- 2023: 5 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 5 · 2018: 5 · 2017: 4 · 2016: 8 · 2015: 3 · 2014: 2 · 2013: 6 · 2012: 5
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Keywords
- transgenic tobacco, apical dominance, root growth, vascular differentiation, plant hormone signaling
- MeSH terms
- Indoleacetic Acids, Peptide Synthases, Phenotype, Plants, Toxic, Pseudomonas, Nicotiana, Transformation, Genetic, Gene Expression
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