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ATP-dependent conjugation of reticulocyte proteins with the polypeptide required for protein degradation.
Open accessAbstract
The heat-stable polypeptide (APF-1) required for ATP-dependent proteolysis in reticulocytes enters into high molecular weight conjugates upon incubation with the fraction of reticulocytes that is retained by DEAE-cellulose. Conjugate formation requires ATP and Mg2+ and its inhibited by N-ethylmaleimide. UTP and GTP are inactive. These properties are identical to those of ATP-dependent protein breakdown in the same system, suggesting that the conjugates are intermediates in this process. The APF-1 conjugates are stable in sodium dodecyl sulfate/polyacrylamide gel electrophoresis and Sephadex G-75 isolation and are resistant to mild acid, alkali, heat denaturation, and reduction; the conjugates are therefore covalent.
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Citations
Cited by
Hershko, A., & Ciechanover, A. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.425 · Cited by 8,840
Self-citationProtein degradation and protection against misfolded or damaged proteins.
Goldberg, A. L. (2003) · Nature · doi:10.1038/nature02263 · Cited by 2,088
Ubiquitin signalling in the NF-κB pathway
Chen, Z. J. (2005) · Nature Cell Biology · doi:10.1038/ncb0805-758 · Cited by 1,184
Proteolysis: from the lysosome to ubiquitin and the proteasome
Ciechanover, A. (2005) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1552 · Cited by 1,133
Self-citationHershko, A., Ciechanover, A., & Varshavsky, A. (2000) · Nature Medicine · doi:10.1038/80384 · Cited by 649
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Metadata
- Authors
- Ciechanover, A., Heller, H., Elias, S., Haas, A. L., & Hershko, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1980-03
- Volume
- 77
- Issue
- 3
- Pages
- 1365-1368
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2034941135
- Cited by
- 562
- References
- 0
- FWCI
- 6.97
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: ATP-dependent conjugation of reticulocyte proteins with the polypeptide required for protein degradation. https://app.cochat.ai/papers/10.1073/pnas.77.3.1365
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- Ciechanover, A., Heller, H., Elias, S., Haas, A. L., & Hershko, A. (1980). ATP-dependent conjugation of reticulocyte proteins with the polypeptide required for protein degradation. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.77.3.1365
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