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Long-term culture of human bone marrow cells.
Open accessAbstract
A method has been described for the long-term culture of human bone marrow cells in liquid medium. Hematopoiesis, as measured by the production of granulocytic-macrophage progenitor cells (CFUc), continued for at least 20 weeks and was dependent upon the presence of a marrow-derived adherent layer of cells. As in the case of murine marrow liquid cultures, the adherent layer consisted of mononuclear phagocytic cells, endothelial cells, and lipid-laden adipocytes, the latter being essential for long-term hematopoiesis. Optimal growth conditions included McCoy's medium supplemented with fetal bovine serum, horse serum, and hydrocortisone and incubation at 33 degrees C. Horse serum in conjunction with hydrocortisone appeared essential for the growth of adipocytes.
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Citations
Cited by
Chaudhary, P. M., & Roninson, I. B. (1991) · Cell · doi:10.1016/0092-8674(91)90141-k · Cited by 934
Hematopoiesis: a human perspective
Doulatov, S., Notta, F., Laurenti, E., & Dick, J. E. (2012) · Cell stem cell · doi:10.1016/j.stem.2012.01.006 · Cited by 895
Minguell, J. J., Erices, A., & Conget, P. (2001) · Experimental Biology and Medicine · doi:10.1177/153537020122600603 · Cited by 871
Regulation of hemopoiesis by bone marrow stromal cells and their products
Dorshkind, K. (1990) · Annual Review of Immunology · doi:10.1146/annurev.iy.08.040190.000551 · Cited by 504
Bone Marrow Mesenchymal Stem Cells: Historical Overview and Concepts
Charbord, P. (2010) · Human Gene Therapy · doi:10.1089/hum.2010.115 · Cited by 441
Metadata
- Authors
- Gartner, S., & Kaplan, H. S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1980-08
- Volume
- 77
- Issue
- 8
- Pages
- 4756-4759
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2090529961
- Cited by
- 588
- References
- 0
- FWCI
- 9.16
- Type
- journal article
- Language
- en
- Open access
- green
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- Gartner, S., & Kaplan, H. S. (1980). Long-term culture of human bone marrow cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.77.8.4756
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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