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pH homeostasis in Escherichia coli: measurement by 31P nuclear magnetic resonance of methylphosphonate and phosphate
Abstract
The intracellular pH of Escherichia coli cells, respiring on endogenous energy sources, was monitored continuously by 31P NMR over an extracellular pH range between 5.5 and 9. pH homeostasis was found to be good over the entire range, with the data conforming to the simple relationship intracellular pH = 7.6 + 0.1(external pH - 7.6) so that the extreme values observed for intracellular pH were 7.4 and 7.8 at external pH 5.5 and 9, respectively. As well as inorganic phosphate, we employed the pH-sensitive NMR probe methylphosphonate, which was taken up by glycerol-grown cells and was nontoxic; its pKa of 7.65 made it an ideal probe for measurement of cytoplasmic pH and alkaline external pH.
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The ratio of cytoplasmic to extracellular F− is numerically equal to the extracellular to cytoplasmic proton activity ratio over two orders of magnitude.
“The ratio of cytoplasmic to extracellular F− is numerically equal to the extracellular to cytoplasmic proton activity ratio over two orders of magnitude (Fig. 4 A), assuming that cytoplasmic pH is regulated near neutrality under these conditions, as has been long known to be the case (Slonczewski et al., 1981; Zilberstein et al., 1984; Wilks and Slonczewski, 2007).”
From Bacterial fluoride resistance, Fluc channels, and the weak acid accumulation effect (2014) · cites this paper as Slonczewski et al., 1981
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Characterization of the cytoplasm of Escherichia coli K-12 as a function of external osmolarity
Cayley, S., Lewis, B. A., Guttman, H. J., & Record, M. T. (1991) · Journal of Molecular Biology · doi:10.1016/0022-2836(91)90212-o · Cited by 486
Bacterial fluoride resistance, Fluc channels, and the weak acid accumulation effect
Ji, C., Stockbridge, R. B., & Miller, C. (2014) · Journal of General Physiology · doi:10.1085/jgp.201411243 · Cited by 97
Native mass spectrometry-based metabolomics identifies metal-binding compounds
Aron, A. T., Petras, D., Schmid, R., Gauglitz, J. M., Büttel, I., Antelo, L., Zhi, H., Nuccio, S.-P., Saak, C. C., Malarney, K. P., et al. (2022) · Nature Chemistry · doi:10.1038/s41557-021-00803-1 · Cited by 79
Metadata
- Authors
- Slonczewski, J. L., Rosen, B. P., Alger, J. R., & Macnab, R. M.
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1981-10
- Volume
- 78
- Issue
- 10
- Pages
- 6271-6275
- ISSN
- 0027-8424
- 1091-6490
- Cited by
- 301
- References
- 0
- Language
- en
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