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Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.
Open accessAbstract
We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human beta 2-microglobulin. Labeled oligonucleotides were used as hybridization probes to screen bacterial clones containing cDNA sequences primed with oligo(dT) and inserted into the plasmid vector pBR322. One beta 2-microglobulin cDNA clone was detected in the 535 bacterial plasmid clones that were screened. The clone has been characterized by blotting and nucleotide sequence analysis. The cloned beta 2-microglobulin sequence contains 217 base pairs of the 3' untranslated region of the mRNA and 328 base pairs (97%) of the coding region.
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Citations
Cited by
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Easy identification of cDNA clones.
Rüther, U., & Müller-Hill, B. (1983) · The EMBO Journal · doi:10.1002/j.1460-2075.1983.tb01659.x · Cited by 573
Detection of sickle cell beta S-globin allele by hybridization with synthetic oligonucleotides.
Conner, B. J., Reyes, A. A., Morin, C., Itakura, K., Teplitz, R. L., & Wallace, R. B. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.1.278 · Cited by 531
Self-citationGlucocorticoid stimulates expression of corticotropin-releasing hormone gene in human placenta.
Robinson, B. G., Emanuel, R. L., Frim, D. M., & Majzoub, J. A. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.14.5244 · Cited by 440
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Metadata
- Authors
- Suggs, S. V., Wallace, R. B., Hirose, T., Kawashima, E. H., & Itakura, K.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1981-11
- Volume
- 78
- Issue
- 11
- Pages
- 6613-6617
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1965011234
- Cited by
- 417
- References
- 0
- FWCI
- 13.54
- Type
- journal article
- Language
- en
- Open access
- green
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- Suggs, S. V., Wallace, R. B., Hirose, T., Kawashima, E. H., & Itakura, K. (1981). Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.78.11.6613
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