The DOI matches its Crossref record.
Growth of a pure population of mouse mast cells in vitro with conditioned medium derived from concanavalin A-stimulated splenocytes.
Open accessAbstract
A pure population of mast cells was obtained after 14 days of culturing mouse bone marrow cells in the presence of medium derived from concanavalin A-stimulated mouse spleen cells. The cells were characterized as mast cells by their morphologic appearance and histologic staining, by their histamine content (450 ng per 10(6) cells) and by the demonstration of IgE receptors on their surface (150,000--440,000 receptor sites per cell). The histamine content and the number of IgE receptors remained constant for at least 7 wk of culture. These mast cells could be passively sensitized to mice hybridoma IgE. They then released 43% of their histamine content upon incubation with anti-mouse hybridoma IgE.
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Citations
Cited by
Galli, S. J. (1990) · Pathology Reviews • 1990 · doi:10.1007/978-1-4612-0485-5_5 · Cited by 615
Wodnar-Filipowicz, A., Heusser, C. H., & Moroni, C. (1989) · Nature · doi:10.1038/339150a0 · Cited by 480
Raposo, G., Tenza, D., Mecheri, S., Peronet, R., Bonnerot, C., & Desaymard, C. (1997) · Molecular Biology of the Cell · doi:10.1091/mbc.8.12.2631 · Cited by 406
Razin, E., Ihle, J. N., Seldin, D., Mencia-Huerta, J. M., Katz, H. R., LeBlanc, P. A., Hein, A., Caulfield, J. P., Austen, K. F., & Stevens, R. L. (1984) · The Journal of Immunology · doi:10.4049/jimmunol.132.3.1479 · Cited by 379
Self-citationNakano, T., Sonoda, T., Hayashi, C., Yamatodani, A., Kanayama, Y., Yamamura, T., Asai, H., Yonezawa, T., Kitamura, Y., & Galli, S. J. (1985) · The Journal of experimental medicine · doi:10.1084/jem.162.3.1025 · Cited by 362
Metadata
- Authors
- Razin, E., Cordon-Cardo, C., & Good, R. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1981-04
- Volume
- 78
- Issue
- 4
- Pages
- 2559-2561
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2003616583
- Cited by
- 234
- References
- 0
- FWCI
- 5.56
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Growth of a pure population of mouse mast cells in vitro with conditioned medium derived from concanavalin A-stimulated splenocytes. https://app.cochat.ai/papers/10.1073/pnas.78.4.2559
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- Razin, E., Cordon-Cardo, C., & Good, R. A. (1981). Growth of a pure population of mouse mast cells in vitro with conditioned medium derived from concanavalin A-stimulated splenocytes. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.78.4.2559
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
