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Cited by 271

The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.

"Nonrandom" DNA sequence analysis in bacteriophage M13 by the dideoxy chain-termination method.

Open access
Poncz, M, Solowiejczyk, D, Ballantine, M, Schwartz, E, Surrey, S·1982·Proceedings of the National Academy of Sciences

Abstract

We describe a rapid "nonrandom" DNA sequence analysis procedure that facilitates the nucleotide sequence determination of large contiguous regions of DNA. The method consists of cloning a restriction endonuclease fragment of interest into bacteriophage M13 followed by construction of a series of nuclease BAL-31 deletion mutants originating from a single site in M13 that is close to the DNA insert. Determination of the size of the deletion mutant is accomplished by hybridization to a complementary single-stranded probe derived from M13 containing that total insert followed by nuclease S1 treatment. Single-stranded M13-insert DNAs of progressively smaller sizes are isolated and analyzed by using a site-specific M13 DNA primer and the dideoxy chain-termination method. In this way, analysis of the DNA sequence proceeds from one end of the total insert to the other in a nonrandom fashion due to generation of a controlled overlapping set of deletion mutants.

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Metadata

Authors
Poncz, M., Solowiejczyk, D., Ballantine, M., Schwartz, E., & Surrey, S.
Venue
Proceedings of the National Academy of Sciences
Published
1982-07
Volume
79
Issue
14
Pages
4298-4302
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W1968058947
Cited by
271
References
14
FWCI
9.49
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: "Nonrandom" DNA sequence analysis in bacteriophage M13 by the dideoxy chain-termination method. https://app.cochat.ai/papers/10.1073/pnas.79.14.4298
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Poncz, M., Solowiejczyk, D., Ballantine, M., Schwartz, E., & Surrey, S. (1982). "Nonrandom" DNA sequence analysis in bacteriophage M13 by the dideoxy chain-termination method. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.79.14.4298

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.