The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine.
Open accessAbstract
We have developed a procedure for simultaneous flow cytometric measurement of cellular DNA content and amount of BrdUrd incorporated into cellular DNA. Propidium iodide was used as a fluorescent probe for total cellular DNA and a monoclonal antibody against BrdUrd was used as a probe for BrdUrd incorporated into DNA. Fluorescein-labeled goat anti-mouse antibody was used to fluorescently label the bound anti-BrdUrd probe. Bivariate DNA/BrdUrd distributions measured for Chinese hamster ovary cells labeled for 30 min with BrdUrd clearly show the G1-and G2M-phase cells to have low BrdUrd-linked fluorescence and the S-phase cells to have high BrdUrd-linked fluorescence. Cell cycle traverse rates were estimated for Chinese hamster ovary cells from bivariate distributions measured for samples taken periodically after pulse labeling with BrdUrd. Bivariate DNA/BrdUrd distributions were also applied in the analysis of the response of C3H murine bone marrow cells to treatment in vivo with 1-beta-D-arabinofuranosylcytosine (araC). Bivariate distributions were measured for bone marrow cells taken from mice that were pulse labeled with BrdUrd at various times after treatment with araC. The resulting DNA/BrdUrd sequences show the kinetics of recovery from araC and allow discrimination of the araC sterilized cells.
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Cites
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Self-citationA fluorescent complex between ethidium bromide and nucleic acids
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Formation of nascent DNA molecules during inhibition of replicon initiation in mammalian cells
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[19] Cell cycle analysis by flow cytometry
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Self-citationAn immunofluorescence method for monitoring DNA synthesis by flow cytometry
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Self-citation
Cited by
The MYC protein activates transcription of the alpha-prothymosin gene.
Eilers, M., Schirm, S., & Bishop, J. M. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb07929.x · Cited by 578
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Self-citationLeuschner, F., Rauch, P. J., Ueno, T., Gorbatov, R., Marinelli, B., Lee, W. W., Dutta, P., Wei, Y., Robbins, C., Iwamoto, Y., et al. (2012) · Journal of Experimental Medicine · doi:10.1084/jem.20111009 · Cited by 521
Review: assessment of cell proliferation in histological material.
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Taxol-induced apoptosis depends on MAP kinase pathways (ERK and p38) and is independent of p53
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Metadata
- Authors
- Dolbeare, F., Gratzner, H., Pallavicini, M. G., & Gray, J. W.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1983-09
- Volume
- 80
- Issue
- 18
- Pages
- 5573-5577
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2032448671
- Cited by
- 957
- References
- 6
- FWCI
- 34.60
- Type
- journal article
- Language
- en
- Open access
- green
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- Dolbeare, F., Gratzner, H., Pallavicini, M. G., & Gray, J. W. (1983). Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.80.18.5573
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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