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Cited by 33Axon Guidance and Neuronal SignalingNeurogenesis and neuroplasticity mechanismsNeuroscience and Neuropharmacology Research

Ultrastructural networks in growth cones and neurites of cultured central nervous system neurons.

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Tsui, H C, Ris, H, Klein, W L·1983·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.80.18.5779PMC PMC384343
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Abstract

We have examined growth cones and neurites of cultured central nervous system neurons by high-voltage electron microscopy. Embryonic chicken retina cells were cultured on polylysine-treated and Formvar-coated gold grids for 2-6 days, fixed, and critical point dried. Growth cones and neurites were examined as unembedded whole mounts. Three-dimensional images from stereo-pair electron micrographs of these regions showed a high degree of ultrastructural articulation, with distinct, non-tapering filaments (5-9 nm in diameter) joining both cytoskeletal and membranous components. In the central regions of growth cones, interconnected structures included microtubules, large membranous sacs (up to 400 nm), and irregular vesicles (25-75 nm). A denser filamentous network was prevalent at the edges of growth cones. This network, which frequently adjoined the surface membrane, linked vesicles of uniform size (35-40 nm). Such vesicles often were seen densely packed in growth cone protrusions that were about the size of small synaptic boutons. Prevalent structural interconnections within growth cones conceivably could play a logistic role in specific membrane assembly, intracellular transport, endocytosis, and secretion. Because such processes are not unique to growth cones, the extensive linkages we have observed may have implications for cytoplasmic structure in general.

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  • Rings of microtubules have been observed in the growth cones of sensory neurons in culture.

    “Rings of microtubules have been observed in the growth cones of sensory neurons in culture (Tsui et al., 1983).”

    From Immunocytochemical localization of tubulin and microtubule-associated protein 2 during the development of hippocampal neurons in culture (1986) · cites this paper as Tsui et al., 1983

    The Abstract reports microtubules in the growth cones of cultured embryonic chicken retina neurons. It does not describe rings of microtubules or identify the studied cells as sensory neurons, so those parts of the claim are unsupported by our copy. (LLM, conf=0.84)

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Research works printed but not linked: 34

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Cited by

Sorted by citations

Authors
Tsui, H. C., Ris, H., & Klein, W. L.
Affiliations
Northwestern University (US)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1983-09
Volume
80
Issue
18
Pages
5779-5783
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.80.18.5779
PMC
PMC384343
OpenAlex
W2035110628
Cited by
33
References
0
Citations by year
2025: 1
FWCI
1.29
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
growth cones, neurites, central nervous system neurons, microtubules, vesicles, cytoskeleton, membrane assembly, intracellular transport, endocytosis, secretion, neuronal ultrastructure
MeSH terms
Animals, Chick Embryo, Cytoskeleton, Microscopy, Electron, Neurons, Retina, Synaptic Vesicles

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