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Cloning and expression of murine immune interferon cDNA.
Open accessAbstract
The murine immune interferon (IFN-gamma) gene was cloned and expressed under control of the simian virus 40 early promoter in the monkey COS-1 cell line. A protein is secreted from these cells having the biological, antigenic, and biochemical characteristics of natural murine IFN-gamma. Cloned murine IFN-gamma cDNAs were obtained by using RNA from both mitogen-induced murine spleens and the transfected COS cells, and both code for identical proteins. The mature murine IFN-gamma encoded is 136 amino acids long, 10 amino acids shorter than human IFN-gamma. The nucleotide homology between the murine and human IFN-gamma genes is 60-65%, whereas the encoded proteins are only 40% homologous. Murine IFN-gamma cDNA was expressed in Escherichia coli under trp promoter control.
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Berger, S. L., & Birkenmeier, C. S. (1979) · Biochemistry · doi:10.1021/bi00590a018 · Cited by 443
Cited by
Schwartz, R. H. (2003) · Annual Review of Immunology · doi:10.1146/annurev.immunol.21.120601.141110 · Cited by 1,487
Heinzel, F. P., Sadick, M. D., Holaday, B. J., Coffman, R. L., & Locksley, R. M. (1989) · The Journal of experimental medicine · doi:10.1084/jem.169.1.59 · Cited by 1,454
Kinashi, T., Harada, N., Severinson, E., Tanabe, T., Sideras, P., Konishi, M., Azuma, C., Tominaga, A., Bergstedt-Lindqvist, S., Takahashi, M., et al. (1986) · Nature · doi:10.1038/324070a0 · Cited by 398
Geiser, A. G., Letterio, J. J., Kulkarni, A. B., Karlsson, S., Roberts, A. B., & Sporn, M. B. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.21.9944 · Cited by 179
Metadata
- Authors
- Gray, P. W., & Goeddel, D. V.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1983-10
- Volume
- 80
- Issue
- 19
- Pages
- 5842-5846
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2013266934
- Cited by
- 446
- References
- 1
- FWCI
- 15.62
- Type
- journal article
- Language
- en
- Open access
- bronze
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