The DOI matches its Crossref record.
Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis.
Open accessAbstract
The Escherichia coli lac operator has been placed on the 3' side of the promoter for the penicillinase gene of Bacillus licheniformis, creating a hybrid promoter controllable by the E. coli lac repressor. The E. coli lac repressor gene has been placed under the control of a promoter and ribosome-binding site that allows expression in Bacillus subtilis. When the penicillinase gene that contains the lac operator is expressed in B. subtilis on a plasmid that also produces the lac repressor, the expression of the penicillinase gene can be modulated by isopropyl beta-D-thiogalactoside (IPTG), an inducer of the lac operon in E. coli. A similar system was constructed from a promoter of the B. subtilis phage SPO-1 and the leukocyte interferon A gene, which allowed the controlled expression of interferon in B. subtilis. These two examples show that a functional control system can be introduced into B. subtilis from E. coli.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Regulation of ribosomal RNA promoters with a synthetic lac operator.
Brosius, J., & Holy, A. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.22.6929 · Cited by 444
Toh, S., Xiong, L., Arias, C. A., Villegas, M. V., Lolans, K., Quinn, J., & Mankin, A. S. (2007) · Molecular Microbiology · doi:10.1111/j.1365-2958.2007.05744.x · Cited by 338
Nakano, M. M., & Zuber, P. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.22.7269-7274.1991 · Cited by 83
Metadata
- Authors
- Yansura, D. G., & Henner, D. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1984-01
- Volume
- 81
- Issue
- 2
- Pages
- 439-443
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2006495190
- Cited by
- 333
- References
- 0
- FWCI
- 5.77
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis. https://app.cochat.ai/papers/10.1073/pnas.81.2.439
- The paper
- Yansura, D. G., & Henner, D. J. (1984). Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.81.2.439
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
