The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Regulation of ribosomal RNA promoters with a synthetic lac operator.
Open accessAbstract
A synthetic 21-base-pair long DNA fragment containing the central lac operator sequence has been inserted near the initiation point of the cloned Escherichia coli rrnB rRNA promoter P2 in the natural and reverse orientation. RNA synthesis is efficiently repressed in both orientations in lac Iq strains and is induced with isopropyl beta-D-thiogalactoside. When the rrnB promoter P1 is also present, upstream from P2 and the synthetic lac operator, repression of transcription is incomplete. The levels of transcription were measured in vivo, indirectly by the expression of a protein (chloramphenicol acetyltransferase), or directly by the expression of a stable RNA (E. coli 4.5S RNA) in a simple assay involving gel electrophoresis of unlabeled total RNA from E. coli. The rrnB promoter constructions can produce high levels of protein expression as well as high levels of expression of stable RNA.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,221
Experiments in molecular genetics
Miller, J. H. (1972) · Medical Entomology and Zoology · Cited by 27,157
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
[57] Sequencing end-labeled DNA with base-specific chemical cleavages
Maxam, A. M., & Gilbert, W. (1980) · Methods in Enzymology · doi:10.1016/s0076-6879(80)65059-9 · Cited by 14,315
Studies on transformation of Escherichia coli with plasmids
Hanahan, D. (1983) · Journal of Molecular Biology · doi:10.1016/s0022-2836(83)80284-8 · Cited by 11,157
Vieira, J., & Messing, J. (1982) · Gene · doi:10.1016/0378-1119(82)90015-4 · Cited by 6,056
Berk, A. J., & Sharp, P. A. (1977) · Cell · doi:10.1016/0092-8674(77)90272-0 · Cited by 2,906
Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli
Brosius, J., Dull, T. J., Sleeter, D. D., & Noller, H. F. (1981) · Journal of Molecular Biology · doi:10.1016/0022-2836(81)90508-8 · Cited by 1,707
Self-citationThe tac promoter: a functional hybrid derived from the trp and lac promoters.
de Boer, H. A., Comstock, L. J., & Vasser, M. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.1.21 · Cited by 1,008
Cited by
A General Method for Site-specific Incorporation of Unnatural Amino Acids into Proteins
Noren, C. J., Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989) · Science · doi:10.1126/science.2649980 · Cited by 1,019
Transcriptional Regulator of Oxidative Stress-Inducible Genes: Direct Activation by Oxidation
Storz, G., Tartaglia, L. A., & Ames, B. N. (1990) · Science · doi:10.1126/science.2183352 · Cited by 874
Construction of a fluorescent biosensor family
De Lorimier, R. M., Smith, J. J., Dwyer, M. A., Looger, L. L., Sali, K. M., Paavola, C. D., Rizk, S. S., Sadigov, S., Conrad, D. W., Loew, L., et al. (2002) · Protein Science · doi:10.1110/ps.021860 · Cited by 332
Itoh, Y., Wang, X., Hinnebusch, B. J., Preston, J. F., & Romeo, T. (2005) · Journal of Bacteriology · doi:10.1128/jb.187.1.382-387.2005 · Cited by 322
Determinants of membrane protein topology.
Boyd, D., Manoil, C., & Beckwith, J. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.23.8525 · Cited by 295
Metadata
- Authors
- Brosius, J., & Holy, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1984-11
- Volume
- 81
- Issue
- 22
- Pages
- 6929-6933
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2013200026
- Cited by
- 444
- References
- 26
- FWCI
- 6.25
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Regulation of ribosomal RNA promoters with a synthetic lac operator. https://app.cochat.ai/papers/10.1073/pnas.81.22.6929
- The paper
- Brosius, J., & Holy, A. (1984). Regulation of ribosomal RNA promoters with a synthetic lac operator. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.81.22.6929
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
