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Cited by 613

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Isolation of brain fibroblast growth factor by heparin-Sepharose affinity chromatography: identity with pituitary fibroblast growth factor.

Open access
Gospodarowicz, D, Cheng, J, Lui, G M, Baird, A, Böhlent, P·1984·Proceedings of the National Academy of Sciences

Abstract

Brain and pituitary fibroblast growth factors (FGF) have been purified to apparent homogeneity from crude tissue extracts by a three-step procedure, including salt precipitation, ion-exchange chromatography, and heparin-Sepharose affinity chromatography. Brain and pituitary FGF have similar amino acid compositions and are indistinguishable with respect to molecular weight (16,000 by polyacrylamide gel electrophoresis), retention behavior in reversed-phase high-performance liquid chromatography, and recognition by antibodies directed against the amino-terminal sequence of pituitary FGF. Brain FGF preparations purified by heparin-Sepharose contain, in addition to the major FGF molecular species, at least two additional forms of the growth factor, which appear to be very similar by all the above criteria, except for retention in high-performance liquid chromatography.

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Crossref · 10.1073/pnas.81.22.6963

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Metadata

Authors
Gospodarowicz, D., Cheng, J., Lui, G. M., Baird, A., & Böhlent, P.
Venue
Proceedings of the National Academy of Sciences
Published
1984-11
Volume
81
Issue
22
Pages
6963-6967
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2002555237
Cited by
613
References
1
FWCI
19.23
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Isolation of brain fibroblast growth factor by heparin-Sepharose affinity chromatography: identity with pituitary fibroblast growth factor. https://app.cochat.ai/papers/10.1073/pnas.81.22.6963
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Gospodarowicz, D., Cheng, J., Lui, G. M., Baird, A., & Böhlent, P. (1984). Isolation of brain fibroblast growth factor by heparin-Sepharose affinity chromatography: identity with pituitary fibroblast growth factor. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.81.22.6963

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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