The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Fibroblast growth factor promotes survival of dissociated hippocampal neurons and enhances neurite extension.
Open accessAbstract
Basic fibroblast growth factor (FGF) has been found to increase neuronal survival and neurite extension in a highly purified population of fetal rat hippocampal neurons under well-defined serum-free cell culture conditions. In the presence of FGF, neuronal survival after 7 days in culture on a simple plastic substrate is increased 4-fold, to 54% of the initial population. Survival is increased 2-fold to 40% on polyornithine-laminin. When FGF was bound to plastic or heparin substrates, neurite outgrowth was significantly increased to lengths comparable to those seen with laminin; however, FGF produced no further increase in neurite outgrowth on laminin. Half-maximal survival was observed at FGF concentrations of about 15 pg/ml (1 pM); half-maximal process outgrowth occurred at about 375 pg/ml (20 pM). The responsive cells were identified as neurons by their labeling with tetanus toxin and by antibodies to neurofilaments and to the neuron-specific enolase. Astrocytes, identified by the presence of glial fibrillary acidic protein, constituted about 10% of cells present at 1 week both in the presence and in the absence of FGF. These results strongly suggest that, in addition to its known mitogenic effects on nonneuronal cells, FGF possesses neurotrophic activity for hippocampal neurons.
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Domingos da Silveira da Luz, A. C., Pereira Dias, G., do Nascimento Bevilaqua, M. C., Cocks, G., Gardino, P. F., Thuret, S., & Nardi, A. E. (2013) · Neuropsychobiology · doi:10.1159/000353269 · Cited by 3,995
Growth of a rat neuroblastoma cell line in serum-free supplemented medium.
Bottenstein, J. E., & Sato, G. H. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.1.514 · Cited by 2,276
Purification of a new neurotrophic factor from mammalian brain.
Barde, Y. A., Edgar, D., & Thoenen, H. (1982) · The EMBO Journal · doi:10.1002/j.1460-2075.1982.tb01207.x · Cited by 1,822
Regressive Events in Neurogenesis
Cowan, W. M., Fawcett, J. W., O'Leary, D. D. M., & Stanfield, B. B. (1984) · Science · doi:10.1126/science.6474175 · Cited by 1,316
Self-citationThe Nerve Growth Factor: Biochemistry, Synthesis, and Mechanism of Action
Greene, L. A., & Shooter, E. M. (1980) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.03.030180.002033 · Cited by 731
Laminin promotes neuritic regeneration from cultured peripheral and central neurons.
Manthorpe, M., Engvall, E., Ruoslahti, E., Longo, F. M., Davis, G. E., & Varon, S. (1983) · The Journal of cell biology · doi:10.1083/jcb.97.6.1882 · Cited by 715
Esch, F., Baird, A., Ling, N., Ueno, N., Hill, F., Denoroy, L., Klepper, R., Gospodarowicz, D., Böhlen, P., & Guillemin, R. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.19.6507 · Cited by 693
Self-citationTrophic Interactions Between Astroglial Cells and Hippocampal Neurons in Culture
Banker, G. A. (1980) · Science · doi:10.1126/science.7403847 · Cited by 674
Edgar, D., Timpl, R., & Thoenen, H. (1984) · The EMBO Journal · doi:10.1002/j.1460-2075.1984.tb01997.x · Cited by 630
Gospodarowicz, D., Cheng, J., Lui, G. M., Baird, A., & Böhlent, P. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.22.6963 · Cited by 613
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Cited by
Trophic factors and neuronal survival
Barde, Y.-A. (1989) · Neuron · doi:10.1016/0896-6273(89)90040-8 · Cited by 1,625
Fibroblast Growth Factor Signaling during Early Vertebrate Development
Böttcher, R. T., & Niehrs, C. (2005) · Endocrine Reviews · doi:10.1210/er.2003-0040 · Cited by 540
Mattson, M., Murrain, M., Guthrie, P., & Kater, S. (1989) · The Journal of Neuroscience · doi:10.1523/jneurosci.09-11-03728.1989 · Cited by 480
Hefti, F., Hartikka, J., & Knusel, B. (1989) · Neurobiology of Aging · doi:10.1016/0197-4580(89)90118-8 · Cited by 466
Lai, C., & Lemke, G. (1991) · Neuron · doi:10.1016/0896-6273(91)90167-x · Cited by 443
Metadata
- Authors
- Walicke, P., Cowan, W. M., Ueno, N., Baird, A., & Guillemin, R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1986-05
- Volume
- 83
- Issue
- 9
- Pages
- 3012-3016
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1977311938
- Cited by
- 733
- References
- 29
- FWCI
- 16.44
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Fibroblast growth factor promotes survival of dissociated hippocampal neurons and enhances neurite extension. https://app.cochat.ai/papers/10.1073/pnas.83.9.3012
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- Walicke, P., Cowan, W. M., Ueno, N., Baird, A., & Guillemin, R. (1986). Fibroblast growth factor promotes survival of dissociated hippocampal neurons and enhances neurite extension. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.83.9.3012
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
