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Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells.

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Yokota, T, Lee, F, Rennick, D, Hall, C, Arai, N, Mosmann, T, Nabel, G, Cantor, H, Arai, K·1984·Proceedings of the National Academy of Sciences

Abstract

A cDNA sequence coding for mouse mast-cell growth-factor (MCGF) has been cloned from a cDNA library prepared from mRNA derived from a concanavalin A-activated mouse T-cell clone. Cloning was achieved using the pcD vector that permits expression of cDNA inserts in mammalian cells. The DNA sequence codes for a polypeptide of 166 amino acid residues including a putative signal peptide. The supernatant fluid obtained after transfection of COS-7 monkey cells with the pcD-MCGF plasmid had mouse MCGF activity. The MCGF amino acid sequence between amino acids 33 and 41, deduced from the nucleotide sequence of its cDNA, is identical to the NH2-terminal sequence of the mouse lymphokine, interleukin 3, suggesting that MCGF is related to interleukin 3.

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Metadata

Authors
Yokota, T., Lee, F., Rennick, D., Hall, C., Arai, N., Mosmann, T., Nabel, G., Cantor, H., & Arai, K.
Venue
Proceedings of the National Academy of Sciences
Published
1984-02
Volume
81
Issue
4
Pages
1070-1074
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W1984646339
Cited by
346
References
0
FWCI
21.87
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells. https://app.cochat.ai/papers/10.1073/pnas.81.4.1070
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Yokota, T., Lee, F., Rennick, D., Hall, C., Arai, N., Mosmann, T., Nabel, G., Cantor, H., & Arai, K. (1984). Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.81.4.1070

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