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Enzyme-catalyzed processes in organic solvents.
Open accessAbstract
Three different lipases (porcine pancreatic, yeast, and mold) can vigorously act as catalysts in a number of nearly anhydrous organic solvents. Various transesterification reactions catalyzed by porcine pancreatic lipase in hexane obey Michaelis-Menten kinetics. The dependence of the catalytic activity of the enzyme in organic media on the pH of the aqueous solution from which it was recovered is bell-shaped, with the maximum coinciding with the pH optimum of the enzymatic activity in water. The catalytic power exhibited by the lipases in organic solvents is comparable to that displayed in water. In addition to transesterification, lipases can catalyze several other processes in organic media including esterification, aminolysis, acyl exchange, thiotransesterification, and oximolysis; some of these reactions proceed to an appreciable extent only in nonaqueous solvents.
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Citations
Cited by
Rules for optimization of biocatalysis in organic solvents
Laane, C., Boeren, S., Vos, K., & Veeger, C. (1987) · Biotechnology and Bioengineering · doi:10.1002/bit.260300112 · Cited by 1,641
Enzymatic catalysis in nonaqueous solvents.
Zaks, A., & Klibanov, A. M. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)69054-4 · Cited by 990
Self-citationProduction of acidic lipase by Aspergillus niger in solid state fermentation
Mahadik, N. D., Puntambekar, U. S., Bastawde, K. B., Khire, J. M., & Gokhale, D. V. (2002) · Process Biochemistry · doi:10.1016/s0032-9592(02)00194-2 · Cited by 323
Soares, C. M., Teixeira, V. H., & Baptista, A. M. (2003) · Biophysical Journal · doi:10.1016/s0006-3495(03)74972-8 · Cited by 149
Metadata
- Authors
- Zaks, A., & Klibanov, A. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1985-05
- Volume
- 82
- Issue
- 10
- Pages
- 3192-3196
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2035176560
- Cited by
- 1,235
- References
- 0
- FWCI
- 7.59
- Type
- journal article
- Language
- en
- Open access
- green
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