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Maintenance of differentiated rat hepatocytes in primary culture.
Open accessAbstract
Normal adult rat hepatocytes remained viable and functional for at least 43 days when plated on collagen-coated dishes and fed chemically defined medium supplemented with dimethyl sulfoxide (Me2SO). Hepatocytes isolated by collagenase perfusion and cultured in the presence or absence of Me2SO were (i) examined by light and electron microscopy for morphological changes; (ii) analyzed for the production of albumin and other plasma proteins; and (iii) tested by autoradiography for DNA synthesis. Me2SO-treated cells continued to produce specific plasma proteins during the entire culture period; albumin production was consistently high (11-19 micrograms/ml of culture medium per 24 hr) from day 2 to at least day 43 after plating. Ultrastructural analyses demonstrated that Me2SO-treated hepatocytes resembled those from intact liver in organization of cytoplasmic organelles and cellular junctions. The optimal concentration for observing the morphological and biochemical effects of Me2SO was 2% (vol/vol). We conclude that supplementation of chemically defined medium with Me2SO enables maintenance of differentiated hepatocytes in culture for extended periods of time.
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Cited by
Godoy, P., Hewitt, N. J., Albrecht, U., Andersen, M. E., Ansari, N., Bhattacharya, S., Bode, J. G., Bolleyn, J., Borner, C., Böttger, J., et al. (2013) · Archives of Toxicology · doi:10.1007/s00204-013-1078-5 · Cited by 1,280
Santos, N. C., Figueira-Coelho, J., Martins-Silva, J., & Saldanha, C. (2003) · Biochemical Pharmacology · doi:10.1016/s0006-2952(03)00002-9 · Cited by 648
Cell and tissue engineering for liver disease
Bhatia, S. N., Underhill, G. H., Zaret, K. S., & Fox, I. J. (2014) · Science Translational Medicine · doi:10.1126/scitranslmed.3005975 · Cited by 309
Hepatocytes in collagen sandwich: evidence for transcriptional and translational regulation.
Dunn, J. C., Tompkins, R. G., & Yarmush, M. L. (1992) · The Journal of cell biology · doi:10.1083/jcb.116.4.1043 · Cited by 295
Potential hepatic stem cells reside in EpCAM+ cells of normal and injured mouse liver
Okabe, M., Tsukahara, Y., Tanaka, M., Suzuki, K., Saito, S., Kamiya, Y., Tsujimura, T., Nakamura, K., & Miyajima, A. (2009) · Development · doi:10.1242/dev.031369 · Cited by 287
Metadata
- Authors
- Isom, H. C., Secott, T., Georgoff, I., Woodworth, C., & Mummaw, J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1985-05
- Volume
- 82
- Issue
- 10
- Pages
- 3252-3256
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2069564134
- Cited by
- 358
- References
- 0
- FWCI
- 17.21
- Type
- journal article
- Language
- en
- Open access
- green
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- Isom, H. C., Secott, T., Georgoff, I., Woodworth, C., & Mummaw, J. (1985). Maintenance of differentiated rat hepatocytes in primary culture. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.82.10.3252
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