The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Cloning and expression in Escherichia coli of the cDNA for murine tumor necrosis factor.
Open accessAbstract
A murine tumor necrosis factor (MuTNF) cDNA was isolated from a cDNA library prepared by using mRNA from the murine macrophage-like cell line PU5-1.8 induced with 4 beta-phorbol 12 beta-myristate 13 alpha-acetate. The cDNA encodes a polypeptide consisting of a 79 amino acid pre sequence followed by a mature MuTNF sequence of 156 amino acids. The 235 amino acid murine pre-TNF polypeptide is 79% homologous to the human pre-TNF protein. There is one potential N-linked glycosylation site on MuTNF, in contrast to human TNF, which lacks any such site. The MuTNF cDNA, when engineered for expression in Escherichia coli, was found to direct the synthesis of biologically active MuTNF as determined by its cytotoxicity against several transformed cell lines.
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Citations
Cites
An endotoxin-induced serum factor that causes necrosis of tumors
Carswell, E. A., Old, L. J., Kassel, R. L., Green, S., Fiore, N., & Williamson, B. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.9.3666 · Cited by 4,532
Cited by
TNF-mediated inflammatory disease
Bradley, Jr. (2008) · The Journal of Pathology · doi:10.1002/path.2287 · Cited by 2,094
Stein, M., Keshav, S., Harris, N., & Gordon, S. (1992) · The Journal of experimental medicine · doi:10.1084/jem.176.1.287 · Cited by 1,960
Cachectin and tumour necrosis factor as two sides of the same biological coin
Beutler, B., & Cerami, A. (1986) · Nature · doi:10.1038/320584a0 · Cited by 1,647
Induction of manganous superoxide dismutase by tumor necrosis factor: possible protective mechanism
Wong, G. H. W., & Goeddel, D. V. (1988) · Science · doi:10.1126/science.3263703 · Cited by 914
Self-citationThe purification of eukaryotic polypeptides synthesized in Escherichia coli
Marston, F. A. (1986) · Biochemical Journal · doi:10.1042/bj2400001 · Cited by 836
Metadata
- Authors
- Pennica, D., Hayflick, J. S., Bringman, T. S., Palladino, M. A., & Goeddel, D. V.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1985-09
- Volume
- 82
- Issue
- 18
- Pages
- 6060-6064
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2046621497
- Cited by
- 390
- References
- 1
- FWCI
- 12.57
- Type
- journal article
- Language
- en
- Open access
- green
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- Pennica, D., Hayflick, J. S., Bringman, T. S., Palladino, M. A., & Goeddel, D. V. (1985). Cloning and expression in Escherichia coli of the cDNA for murine tumor necrosis factor. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.82.18.6060
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
