Identification of the components necessary for adenovirus translational control and their utilization in cDNA expression vectors
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A transient expression system was used to study the role of the adenovirus late and simian virus 40 (SV40) early mRNA leader sequences and adenovirus virus-associated (VA) RNAs in mRNA translation. Hybrid transcription units containing the adenovirus late and SV40 early promoters fused to various coding regions were introduced into monkey COS cells on plasmids containing a SV40 origin of replication. The translational efficiencies of the mRNAs produced from these plasmids were determined after alterations in the viral leader sequences or in the presence of VA RNAs provided by adenovirus infection of the transfected cells or by cotransfection with plasmids containing the VA genes. Efficient translation of mRNA with either adenovirus or SV40 leader sequences is dependent upon the presence of VA RNA. Translational stimulation by VA RNA of mRNAs containing the adenovirus tripartite leader sequences is dramatically reduced if leader exons 2 and 3 are removed or if their orientation is altered. Sequence analysis has indicated a homology between the nontranslated 5' end of SV40 early mRNA and sequences at the border of the 2nd and 3rd tripartite leader exons, which may be responsible for the increased translation of these mRNAs in the presence of VA RNA.
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Adenovirus TPL can enhance protein expression by enhancing translation initiation.
“Some regulatory elements, such as adenovirus TPL (tripartite leader), can enhance protein expression by enhancing translation initiation [15].”
From Untranslated Region Sequences and the Efficacy of mRNA Vaccines against Tuberculosis (2024) · cites this paper as [15]
TPL contains an internal ribosome entry site enabling it to recruit a ribosome independently of interactions with the mRNA 5′ 7-methyl G cap.
“TPL contains an internal ribosome entry site enabling it to recruit a ribosome independently of interactions with the mRNA 5′ 7-methyl G cap.”
From Untranslated Region Sequences and the Efficacy of mRNA Vaccines against Tuberculosis (2024) · cites this paper as [15]
TPL is a nucleotide sequence in the 5′UTR of adenovirus late mRNAs and has previously been thought to initiate translation of late adenoviral mRNAs in a cap-independent manner.
“TPL is a nucleotide sequence in the 5′UTR of adenovirus late mRNAs and has previously been thought to initiate translation of late adenoviral mRNAs in a cap-independent manner [15,36].”
From Untranslated Region Sequences and the Efficacy of mRNA Vaccines against Tuberculosis (2024) · cites this paper as [15]
In prior studies, the 5′UTR sequence of TPL consisted of 245 nucleotides and had a complex secondary structure.
“The 5′UTR sequence of TPL consisted of 245 nucleotides in those studies and had a complex secondary structure.”
From Effects of Combinations of Untranslated-Region Sequences on Translation of mRNA (2023) · cites this paper as [44]
The adenovirus tripartite leader (TPL) functions as an enhancer for translation of viral late-gene mRNAs and is thought to initiate translation of late adenoviral mRNAs in a cap-independent manner.
“The tripartite leader (TPL) of the adenovirus is known to function as an enhancer for translation of viral late-gene mRNAs and is thought to have an ability to initiate translation of late adenoviral mRNAs in a cap-independent manner [43,44].”
From Effects of Combinations of Untranslated-Region Sequences on Translation of mRNA (2023) · cites this paper as [44]
TPL contains an IRES that allows it to recruit a ribosome independently of interactions with the 5′ 7-methyl G cap of mRNA.
“This research [43,44] indicates that TPL contains an IRES due to which TPL is capable of recruiting a ribosome, independently of interactions with the 5′ 7-methyl G cap of mRNA.”
From Effects of Combinations of Untranslated-Region Sequences on Translation of mRNA (2023) · cites this paper as [44]
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Untranslated Region Sequences and the Efficacy of mRNA Vaccines against Tuberculosis
Reshetnikov, V., Terenin, I., Shepelkova, G., Yeremeev, V., Kolmykov, S., Nagornykh, M., Kolosova, E., Sokolova, T., Zaborova, O., Kukushkin, I., et al. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25020888 · Cited by 33 · see citing works
Effects of Combinations of Untranslated-Region Sequences on Translation of mRNA
Kirshina, A., Vasileva, O., Kunyk, D., Seregina, K., Muslimov, A., Ivanov, R., & Reshetnikov, V. (2023) · Biomolecules · doi:10.3390/biom13111677 · Cited by 26 · see citing works
- Authors
- Kaufman, R. J.
- Corresponding author
- Kaufman, R J
- ORCID
- Kaufman, R J
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1985-02
- Volume
- 82
- Issue
- 3
- Pages
- 689-693
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC397111
- OpenAlex
- W1968475920
- Cited by
- 135
- References
- 0
- Citations by year
- 2024: 1 · 2023: 4 · 2020: 1 · 2018: 1 · 2017: 1
- FWCI
- 6.78
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Keywords
- adenovirus, translational regulation, virus-associated RNA, mRNA leader sequence, transient expression system, COS cells, cotransfection, cDNA expression vector, translation efficiency
- MeSH terms
- Acetyltransferases, Adenoviridae, Animals, Haplorhini, DNA, Gene Expression Regulation, Interferon-gamma, Operon, Pentosyltransferases, Plasmids, Simian virus 40, Tetrahydrofolate Dehydrogenase, Transfection, Protein Biosynthesis, Chloramphenicol O-Acetyltransferase
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