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Polynucleotide phosphorylase and ribonuclease II are required for cell viability and mRNA turnover in Escherichia coli K-12.
Open accessAbstract
The isolation of a temperature-sensitive allele of RNase II (rnb) by in vitro mutagenesis has permitted the demonstration that RNase II and polynucleotide phosphorylase (PNPase) are required for cell viability and mRNA turnover in Escherichia coli. Double-mutant strains carrying the pnp-7 and rnb-500 alleles (PNPase deficient and RNase II thermolabile) ceased growing in Luria broth within 30 min after shift to the nonpermissive temperature. Cessation of growth was accompanied by an accumulation of mRNA fragments 100-1500 nucleotides long. In contrast, single-mutant and wild-type control strains grew normally at the nonpermissive temperature and did not accumulate mRNA. No significant changes in rRNA patterns were observed in any of the strains.
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Cited by
The critical role of RNA processing and degradation in the control of gene expression
Arraiano, C. M., Andrade, J. M., Domingues, S., Guinote, I. B., Malecki, M., Matos, R. G., Moreira, R. N., Pobre, V., Reis, F. P., Saramago, M., et al. (2010) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2010.00242.x · Cited by 349
Messenger RNA Stability and Its Role in Control of Gene Expression in Bacteria and Phages
Grunberg-Manago, M. (1999) · Annual Review of Genetics · doi:10.1146/annurev.genet.33.1.193 · Cited by 285
Lightowlers, R. N., Rozanska, A., & Chrzanowska-Lightowlers, Z. M. (2014) · FEBS Letters · doi:10.1016/j.febslet.2014.05.054 · Cited by 70
Metadata
- Authors
- Donovan, W. P., & Kushner, S. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1986-01
- Volume
- 83
- Issue
- 1
- Pages
- 120-124
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1988098435
- Cited by
- 430
- References
- 0
- FWCI
- 7.60
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Polynucleotide phosphorylase and ribonuclease II are required for cell viability and mRNA turnover in Escherichia coli K-12. https://app.cochat.ai/papers/10.1073/pnas.83.1.120
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- Donovan, W. P., & Kushner, S. R. (1986). Polynucleotide phosphorylase and ribonuclease II are required for cell viability and mRNA turnover in Escherichia coli K-12. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.83.1.120
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