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Characterization of leukotriene A4 synthase from murine mast cells: evidence for its identity to arachidonate 5-lipoxygenase.
Open accessAbstract
Leukotriene A4 synthase was purified from the cytosolic fraction of murine mast cells. The enzyme converted 5-hydroperoxy-6-trans-8,11,14-cis-icosatetraenoic acid (5-HPETE) to leukotriene A4. This unstable product was identified by demonstration of two epimers of 6-transleukotriene B4, methanol trapping, as well as further transformation to leukotriene B4 by leukotriene A4 hydrolase. Leukotriene A4 synthase stereospecifically eliminated the D-hydrogen at C-10 (pro-R) in the synthesis of leukotriene A4 when incubated with [10D-3H;3-(14)C]5-HPETE. The purified enzyme also exhibited 5-lipoxygenase activity toward arachidonic acid and 8-lipoxygenase activity towards 8,11,14-cisicosatrienoic acid. All of these activities required Ca2+ and ATP for their maximal velocities. The effects of heat treatment and of several lipoxygenase inhibitors on these enzyme activities as well as coelution in various chromatographic systems strongly suggest that lipoxygenase and leukotriene A4 synthase activities reside in the same enzyme molecule.
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Citations
Cited by
Lipoxygenase and leukotriene pathways: biochemistry, biology, and roles in disease
Haeggström, J. Z., & Funk, C. D. (2011) · Chemical Reviews · doi:10.1021/cr200246d · Cited by 875
Rouzer, C. A., Ford-Hutchinson, A. W., Morton, H. E., & Gillard, J. W. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)40034-3 · Cited by 314
Metadata
- Authors
- Shimizu, T., Izumi, T., Seyama, Y., Tadokoro, K., Rådmark, O., & Samuelsson, B.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1986-06
- Volume
- 83
- Issue
- 12
- Pages
- 4175-4179
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2089073520
- Cited by
- 173
- References
- 0
- FWCI
- 9.21
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Characterization of leukotriene A4 synthase from murine mast cells: evidence for its identity to arachidonate 5-lipoxygenase. https://app.cochat.ai/papers/10.1073/pnas.83.12.4175
- The paper
- Shimizu, T., Izumi, T., Seyama, Y., Tadokoro, K., Rådmark, O., & Samuelsson, B. (1986). Characterization of leukotriene A4 synthase from murine mast cells: evidence for its identity to arachidonate 5-lipoxygenase. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.83.12.4175
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