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Two related but distinct forms of the Mr 36,000 tyrosine kinase substrate (calpactin) that interact with phospholipid and actin in a Ca2+-dependent manner.
Open accessAbstract
A method was devised that allows the identification of proteins related to the Mr 36,000 tyrosine kinase substrate calpactin based on their ability to interact with actin and phospholipid in a calcium-dependent manner. Two distinct proteins, detected in human A431 cells and fibroblasts, were resolved by two-dimensional gel electrophoresis. One of these proteins (calpactin I) appears identical to the Mr 34,000-39,000 substrate of the pp60src tyrosine kinase and the second (calpactin II) reacts with antibodies to the Mr 35,000 substrate of the epidermal growth factor receptor. Both proteins interact with phospholipid and actin, are rather basic, and share structural and antigenic determinants. A major difference between the two proteins is noted in their state of association with the Mr 10,000 light chain; i.e., calpactin I is associated with the light chain while calpactin II is not.
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Citations
Cited by
Donato, R. (2001) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/s1357-2725(01)00046-2 · Cited by 1,580
Reynolds, A. B., Roesel, D. J., Kanner, S. B., & Parsons, J. T. (1989) · Molecular and Cellular Biology · doi:10.1128/mcb.9.2.629 · Cited by 342
Blackwood, R. A., & Ernst, J. D. (1990) · Biochemical Journal · doi:10.1042/bj2660195 · Cited by 290
Metadata
- Authors
- Glenney, J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1986-06
- Volume
- 83
- Issue
- 12
- Pages
- 4258-4262
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2026581597
- Cited by
- 191
- References
- 0
- FWCI
- 14.71
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Two related but distinct forms of the Mr 36,000 tyrosine kinase substrate (calpactin) that interact with phospholipid and actin in a Ca2+-dependent manner. https://app.cochat.ai/papers/10.1073/pnas.83.12.4258
- The paper
- Glenney, J. (1986). Two related but distinct forms of the Mr 36,000 tyrosine kinase substrate (calpactin) that interact with phospholipid and actin in a Ca2+-dependent manner. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.83.12.4258
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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