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Protein sequencing by tandem mass spectrometry.
Open accessAbstract
Methodology for determining amino acid sequences of proteins by tandem mass spectrometry is described. The approach involves enzymatic and/or chemical degradation of the protein to a collection of peptides which are then fractionated by high-performance liquid chromatography. Each fraction, containing as many as 10-15 peptides, is then analyzed directly, without further purification, by a combination of liquid secondary-ion/collision-activated dissociation mass spectrometry on a multianalyzer instrument. Interpretation of collision-activated dissociation mass spectra is described, and results are presented from a study of soluble peptides produced by treatment of apolipoprotein B with cyanogen bromide and trypsin.
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Cited by
Muzio, M., Chinnaiyan, A. M., Kischkel, F. C., O'Rourke, K., Shevchenko, A., Ni, J., Scaffidi, C., Bretz, J. D., Zhang, M., Gentz, R., et al. (1996) · Cell · doi:10.1016/s0092-8674(00)81266-0 · Cited by 3,046
Protein Analysis by Shotgun/Bottom-up Proteomics
Zhang, Y., Fonslow, B. R., Shan, B., Baek, M.-C., & Yates, J. R. (2013) · Chemical Reviews · doi:10.1021/cr3003533 · Cited by 1,491
Self-citationTrypsin Cleaves Exclusively C-terminal to Arginine and Lysine Residues
Olsen, J. V., Ong, S.-E., & Mann, M. (2004) · Molecular & Cellular Proteomics · doi:10.1074/mcp.t400003-mcp200 · Cited by 1,254
Lu, P., Vogel, C., Wang, R., Yao, X., & Marcotte, E. M. (2007) · Nature Biotechnology · doi:10.1038/nbt1270 · Cited by 1,139
New developments in biochemical mass spectrometry: electrospray ionization
Smith, R. D., Loo, J. A., Edmonds, C. G., Barinaga, C. J., & Udseth, H. R. (1990) · Analytical Chemistry · doi:10.1021/ac00208a002 · Cited by 1,051
Metadata
- Authors
- Hunt, D. F., Yates, J. R., Shabanowitz, J., Winston, S., & Hauer, C. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1986-09
- Volume
- 83
- Issue
- 17
- Pages
- 6233-6237
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1981978551
- Cited by
- 1,180
- References
- 0
- FWCI
- 18.86
- Type
- journal article
- Language
- en
- Open access
- green
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- Hunt, D. F., Yates, J. R., Shabanowitz, J., Winston, S., & Hauer, C. R. (1986). Protein sequencing by tandem mass spectrometry. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.83.17.6233
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