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beta-Glucuronidase from Escherichia coli as a gene-fusion marker.
Open accessAbstract
We have developed a gene-fusion system based on the Escherichia coli beta-glucuronidase gene (uidA). The uidA gene has been cloned from E. coli K-12 and its entire nucleotide sequence has been determined. beta-Glucuronidase has been purified to homogeneity and characterized. The enzyme has a subunit molecular weight of 68,200, is very stable, and is easily and sensitively assayed using commercially available substrates. We have constructed gene fusions of the E. coli lacZ promoter and coding region with the coding region of the uidA gene that show beta-glucuronidase activity under lac control. Plasmid vectors have been constructed to facilitate the transfer of the beta-glucuronidase coding region to heterologous control regions, using many different restriction endonuclease cleavage sites. There are several biological systems in which uidA-encoded beta-glucuronidase may be an attractive alternative or complement to previously described gene-fusion markers such as beta-galactosidase or chloramphenicol acetyltransferase.
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Liu, Q., Kasuga, M., Sakuma, Y., Abe, H., Miura, S., Yamaguchi-Shinozaki, K., & Shinozaki, K. (1998) · The Plant Cell · doi:10.1105/tpc.10.8.1391 · Cited by 3,044
Role of arabidopsis MYC and MYB homologs in drought- and abscisic acid-regulated gene expression.
Abe, H., Yamaguchi-Shinozaki, K., Urao, T., Iwasaki, T., Hosokawa, D., & Shinozaki, K. (1997) · The Plant Cell · doi:10.1105/tpc.9.10.1859 · Cited by 1,049
Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes.
Kozak, M. (1987) · Molecular and Cellular Biology · doi:10.1128/mcb.7.10.3438 · Cited by 555
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Fluorogenic and chromogenic substrates used in bacterial diagnostics
Manafi, M., Kneifel, W., & Bascomb, S. (1991) · Microbiological Reviews · doi:10.1128/mmbr.55.3.335-348.1991 · Cited by 277
Metadata
- Authors
- Jefferson, R. A., Burgess, S. M., & Hirsh, D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1986-11
- Volume
- 83
- Issue
- 22
- Pages
- 8447-8451
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1978862919
- Cited by
- 996
- References
- 5
- FWCI
- 12.50
- Type
- journal article
- Language
- en
- Open access
- green
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- Jefferson, R. A., Burgess, S. M., & Hirsh, D. (1986). beta-Glucuronidase from Escherichia coli as a gene-fusion marker. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.83.22.8447
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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