The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
Antiviral effect of an oligo(nucleoside methylphosphonate) complementary to the splice junction of herpes simplex virus type 1 immediate early pre-mRNAs 4 and 5.
Open accessAbstract
Selective inhibition of regulatory immediate early (IE) genes of herpes simplex virus type 1 (HSV-1) should inhibit virus growth. Treatment of HSV-1-infected cells with the oligo(nucleoside methylphosphonate) d(TpCCTCCTG) (deoxynucleoside methylphosphonate residues in italic), which is complementary to the acceptor splice junction of HSV-1 IE pre-mRNA 4 and 5, before (1-24 hr) or at the time of infection caused a dose-dependent inhibition in virus replication. Virus titers were decreased 50% and 90% in cells treated with 25 microM and 75 microM oligomer, respectively; at 300 microM, a 99% reduction in virus production was observed. Viral DNA synthesis was reduced 70-75% and there was a 90% reduction in synthesis of viral proteins, including other IE species and viral functional (130-kDa major DNA-binding) and structural (glycoprotein gB) proteins. In the same concentration range, d(TpCCTCCTG) caused a minimal reduction (0-30%) in protein synthesis and growth rates (less than 40%) of uninfected cells. The data suggest that oligo(nucleoside methylphosphonate)s may be effective in antiviral chemotherapy.
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Metadata
- Authors
- Smith, C. C., Aurelian, L., Reddy, M. P., Miller, P. S., & Ts'o, P. O.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1986-05
- Volume
- 83
- Issue
- 9
- Pages
- 2787-2791
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1963871104
- Cited by
- 297
- References
- 16
- FWCI
- 26.84
- Type
- journal article
- Language
- en
- Open access
- green
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- Smith, C. C., Aurelian, L., Reddy, M. P., Miller, P. S., & Ts'o, P. O. (1986). Antiviral effect of an oligo(nucleoside methylphosphonate) complementary to the splice junction of herpes simplex virus type 1 immediate early pre-mRNAs 4 and 5. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.83.9.2787
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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