The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Characterization of oligonucleotide transport into living cells.
Open accessAbstract
Addition of antisense oligonucleotides to cell cultures has been used to specifically inhibit gene expression. We have investigated the mechanism by which oligonucleotides enter living cells. These compounds are taken up by cells in a saturable, size-dependent manner compatible with receptor-mediated endocytosis. Polynucleotides of any length are competitive inhibitors of oligomer transport, providing they possess a 5'-phosphate moiety. Using oligo(dT)-cellulose for affinity purification, we have identified an 80-kDa surface protein that may mediate transport. Knowledge of the oligonucleotide transport mechanism should facilitate the design of more effective synthetic antisense oligomers as potential clinical agents.
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Silverstein, S. C., Steinman, R. M., & Cohn, Z. A. (1977) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.46.070177.003321 · Cited by 1,286
pH and the recycling of transferrin during receptor-mediated endocytosis.
Dautry-Varsat, A., Ciechanover, A., & Lodish, H. F. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.8.2258 · Cited by 1,179
Eckstein, F. (1985) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.54.070185.002055 · Cited by 849
A c-myc antisense oligodeoxynucleotide inhibits entry into S phase but not progress from G0 to G1
Heikkila, R., Schwab, G., Wickstrom, E., Loke, S. L., Pluznik, D. H., Watt, R., & Neckers, L. M. (1987) · Nature · doi:10.1038/328445a0 · Cited by 683
Self-citationJourney to the Center of the Cell: Role of the Receptosome
Pastan, I. H., & Willingham, M. C. (1981) · Science · doi:10.1126/science.6170111 · Cited by 499
van Renswoude, J., Bridges, K. R., Harford, J. B., & Klausner, R. D. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.20.6186 · Cited by 488
Holt, J. T., Redner, R. L., & Nienhuis, A. W. (1988) · Molecular and Cellular Biology · doi:10.1128/mcb.8.2.963 · Cited by 464
Zamecnik, P. C., Goodchild, J., Taguchi, Y., & Sarin, P. S. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.12.4143 · Cited by 400
The receptosome: An intermediate organelle of receptor-mediated endocytosis in cultured fibroblasts
Willingham, M. C., & Pastan, I. (1980) · Cell · doi:10.1016/0092-8674(80)90115-4 · Cited by 391
Wickstrom, E. L., Bacon, T. A., Gonzalez, A., Freeman, D. L., Lyman, G. H., & Wickstrom, E. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.4.1028 · Cited by 369
Cited by
Progress in antisense technology
Crooke, S. T. (2004) · Annual Review of Medicine · doi:10.1146/annurev.med.55.091902.104408 · Cited by 417
Poly(hydroxyalkanoates): A Fifth Class of Physiologically Important Organic Biopolymers?
Müller, H., & Seebach, D. (1993) · Angewandte Chemie International Edition in English · doi:10.1002/anie.199304771 · Cited by 363
Bacterial CpG DNA Activates Immune Cells to Signal Infectious Danger
Wagner, H. (1999) · Advances in Immunology · doi:10.1016/s0065-2776(08)60790-7 · Cited by 314
Griffin, L., Tidmarsh, G., Bock, L., Toole, J., & Leung, L. (1993) · Blood · doi:10.1182/blood.v81.12.3271.3271 · Cited by 285
Reversal of Diabetes Insipidus in Brattleboro Rats: Intrahypothalamic Injection of Vasopressin mRNA
Jirikowski, G. F., Sanna, P. P., Maciejewski-Lenoir, D., & Bloom, F. E. (1992) · Science · doi:10.1126/science.1546298 · Cited by 282
Metadata
- Authors
- Loke, S. L., Stein, C. A., Zhang, X. H., Mori, K., Nakanishi, M., Subasinghe, C., Cohen, J. S., & Neckers, L. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1989-05
- Volume
- 86
- Issue
- 10
- Pages
- 3474-3478
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2068230814
- Cited by
- 734
- References
- 22
- FWCI
- 13.14
- Type
- journal article
- Language
- en
- Open access
- green
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- Loke, S. L., Stein, C. A., Zhang, X. H., Mori, K., Nakanishi, M., Subasinghe, C., Cohen, J. S., & Neckers, L. M. (1989). Characterization of oligonucleotide transport into living cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.10.3474
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
