The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
Open accessAbstract
A chemically modified phage T7 DNA polymerase has three properties that make it ideal for DNA sequencing by the chain-termination method. The enzyme is highly processive, catalyzing the polymerization of thousands of nucleotides without dissociating. By virtue of the modification the 3' to 5' exonuclease activity is eliminated. The modified polymerase efficiently uses nucleotide analogs that increase the electrophoretic resolution of bands in gels. Consequently, dideoxynucleotide-terminated fragments have highly uniform radioactive intensity throughout the range of a few to thousands of nucleotides in length. There is virtually no background due to terminations at pause sites or secondary-structure impediments. Processive synthesis with dITP in place of dGTP eliminates band compressions, making possible the unambiguous determination of sequences from a single orientation.
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Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
Cited by
Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs
Eriani, G., Delarue, M., Poch, O., Gangloff, J., & Moras, D. (1990) · Nature · doi:10.1038/347203a0 · Cited by 1,380
A System for Rapid DNA Sequencing with Fluorescent Chain-Terminating Dideoxynucleotides
Prober, J. M., Trainor, G. L., Dam, R. J., Hobbs, F. W., Robertson, C. W., Zagursky, R. J., Cocuzza, A. J., Jensen, M. A., & Baumeister, K. (1987) · Science · doi:10.1126/science.2443975 · Cited by 855
Query, C. C., Bentley, R. C., & Keene, J. D. (1989) · Cell · doi:10.1016/0092-8674(89)90175-x · Cited by 580
Collawn, J. F., Stangel, M., Kuhn, L. A., Esekogwu, V., Jing, S., Trowbridge, I. S., & Tainer, J. A. (1990) · Cell · doi:10.1016/0092-8674(90)90509-d · Cited by 518
Expression cloning and characterization of the canine parietal cell gastrin receptor.
Kopin, A. S., Lee, Y. M., McBride, E. W., Miller, L. J., Lu, M., Lin, H. Y., Kolakowski, L. F., & Beinborn, M. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.8.3605 · Cited by 508
Metadata
- Authors
- Tabor, S., & Richardson, C. C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1987-07
- Volume
- 84
- Issue
- 14
- Pages
- 4767-4771
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2047043554
- Cited by
- 2,241
- References
- 1
- FWCI
- 99.73
- Type
- journal article
- Language
- en
- Open access
- green
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- Tabor, S., & Richardson, C. C. (1987). DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.84.14.4767
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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