The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
A System for Rapid DNA Sequencing with Fluorescent Chain-Terminating Dideoxynucleotides
Abstract
A DNA sequencing system based on the use of a novel set of four chain-terminating dideoxynucleotides, each carrying a different chemically tuned succinylfluorescein dye distinguished by its fluorescent emission is described. Avian myeloblastosis virus reverse transcriptase is used in a modified dideoxy DNA sequencing protocol to produce a complete set of fluorescence-tagged fragments in one reaction mixture. These DNA fragments are resolved by polyacrylamide gel electrophoresis in one sequencing lane and are identified by a fluorescence detection system specifically matched to the emission characteristics of this dye set. A scanning system allows multiple samples to be run simultaneously and computer-based automatic base sequence identifications to be made. The sequence analysis of M13 phage DNA made with this system is described.
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Cites
DNA sequencing with chain-terminating inhibitors
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[57] Sequencing end-labeled DNA with base-specific chemical cleavages
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A new method for sequencing DNA.
Maxam, A. M., & Gilbert, W. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.2.560 · Cited by 7,922
DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
Tabor, S., & Richardson, C. C. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.14.4767 · Cited by 2,241
Fluorescence detection in automated DNA sequence analysis
Smith, L. M., Sanders, J. Z., Kaiser, R. J., Hughes, P., Dodd, C., Connell, C. R., Heiner, C., Kent, S. B. H., & Hood, L. E. (1986) · Nature · doi:10.1038/321674a0 · Cited by 1,714
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.
Langer, P. R., Waldrop, A. A., & Ward, D. C. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.11.6633 · Cited by 1,145
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Cited by
Initial sequencing and analysis of the human genome
International Human Genome Sequencing Consortium, Whitehead Institute for Biomedical Research, C. F. G. R., Lander, E. S., Linton, L. M., Birren, B., Nusbaum, C., Zody, M. C., Baldwin, J., Devon, K., Dewar, K., et al. (2001) · Nature · doi:10.1038/35057062 · Cited by 24,216
The sequence of the human genome
Venter, J. C., Adams, M. D., Myers, E. W., Li, P. W., Mural, R. J., Sutton, G. G., Smith, H. O., Yandell, M., Evans, C. A., Holt, R. A., et al. (2001) · Science · doi:10.1126/science.1058040 · Cited by 13,909
Genome sequencing in microfabricated high-density picolitre reactors
Margulies, M., Egholm, M., Altman, W. E., Attiya, S., Bader, J. S., Bemben, L. A., Berka, J., Braverman, M. S., Chen, Y.-J., Chen, Z., et al. (2005) · Nature · doi:10.1038/nature03959 · Cited by 7,693
The sequence of sequencers: The history of sequencing DNA
Heather, J. M., & Chain, B. (2016) · Genomics · doi:10.1016/j.ygeno.2015.11.003 · Cited by 1,397
Next-Generation Sequencing: Advantages, Disadvantages, and Future
Ari, Ş., & Arikan, M. (2016) · Plant Omics: Trends and Applications · doi:10.1007/978-3-319-31703-8_5 · Cited by 84
Metadata
- Authors
- Prober, J. M., Trainor, G. L., Dam, R. J., Hobbs, F. W., Robertson, C. W., Zagursky, R. J., Cocuzza, A. J., Jensen, M. A., & Baumeister, K.
- Venue
- Science
- Published
- 1987-10-16
- Volume
- 238
- Issue
- 4825
- Pages
- 336-341
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2031928161
- Cited by
- 855
- References
- 12
- FWCI
- 10.57
- Type
- journal article
- Language
- en
- Open access
- closed
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- Prober, J. M., Trainor, G. L., Dam, R. J., Hobbs, F. W., Robertson, C. W., Zagursky, R. J., Cocuzza, A. J., Jensen, M. A., & Baumeister, K. (1987). A System for Rapid DNA Sequencing with Fluorescent Chain-Terminating Dideoxynucleotides. Science. https://doi.org/10.1126/science.2443975
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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