Cited by 368Iron Metabolism and DisordersRNA and protein synthesis mechanismsRNA Research and Splicing

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Iron regulates ferritin mRNA translation through a segment of its 5′-untranslated region.

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Aziz, N, Munro, H N·1987·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.84.23.8478PMC PMC299567

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Abstract

In previous studies, we showed that acute administration of iron to intact rats or to rat hepatoma cells in culture induces synthesis of the iron-storage protein ferritin by activating translation of inactive cytoplasmic ferritin mRNAs for both the heavy (H) and the light (L) subunits. In the course of activation, these ferritin mRNAs are recruited onto polysomes. To elucidate the structural features of these mRNAs involved in the translational response to iron, a chimera was constructed from the 5' and 3' untranslated regions (UTRs) of ferritin L subunit mRNA fused to the reading frame of the mRNA of bacterial chloramphenicol acetyltransferase (CAT). This chimera and deletion constructs derived from it were introduced into a rat hepatoma cell line by retrovirus-mediated gene transfer. The complete chimera showed increased CAT activity in response to iron enrichment of the medium, whereas deletion of the first 67 nucleotides of the 5' UTR, which contain a highly conserved sequence, caused loss of regulation by iron. Whereas cis-acting sequences located in the 5' flanking regions of many genes have been repeatedly implicated in modulating their transcriptional expression, we report here a specific regulatory translational sequence found within the 5' UTR of a eukaryotic mRNA.

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Authors: Aziz, N., & Munro, H. N.
Affiliations: Massachusetts Institute of Technology (US)
ORCID: Aziz, N
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 1987-12
Volume: 84
Issue: 23
Pages: 8478-8482
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.84.23.8478
PMC: PMC299567
OpenAlex: W2032558977
Cited by: 368
References: 28
Citations by year: 2026: 3 · 2025: 8 · 2024: 3 · 2023: 1 · 2022: 2 · 2021: 4 · 2020: 4 · 2019: 3 · 2018: 7 · 2017: 3 · 2016: 6 · 2015: 2 · 2014: 8 · 2013: 5 · 2012: 3
FWCI: 16.87
Type: journal article
Language: en
Open access: green
europepmc.org
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords: iron regulation, ferritin, mRNA translation, 5' untranslated region, translational regulation, polysome association, cis-regulatory elements, hepatoma cells, iron storage
MeSH terms: Animals, Cell Line, DNA Mutational Analysis, Ferritins, Gene Expression Regulation, Iron, Recombinant Fusion Proteins, Regulatory Sequences, Nucleic Acid, RNA, Messenger, Transfection, Protein Biosynthesis, Rats

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Iron regulates ferritin mRNA translation through a segment of its 5′-untranslated region.

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