The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Expression of a synthetic bovine rhodopsin gene in monkey kidney cells.
Open accessAbstract
We report here the high-level expression of a synthetic gene for bovine rhodopsin in transfected monkey kidney COS-1 cells. Rhodopsin is produced in these cells to a level of 0.3% of the cell protein, and it binds exogenously added 11-cis-retinal to generate the characteristic rhodopsin absorption spectrum. We describe a one-step immunoaffinity procedure for purification of the rhodopsin essentially to homogeneity. The COS-1 cell rhodopsin activates the GTPase activity of bovine transducin in a light-dependent manner with the same specific activity as that of purified bovine rhodopsin. Electron microscopy of immunogold-stained cells indicates that rhodopsin is located in the plasma membrane of the transfected cells and is oriented with the amino terminus on the extracellular side of the membrane. This orientation is analogous to that of rhodopsin in the disk membranes of photoreceptor cells in the bovine retina.
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Kaufman, R. J. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.3.689 · Cited by 135
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Cited by
G Protein–Coupled Receptor Rhodopsin
Palczewski, K. (2006) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.75.103004.142743 · Cited by 764
Crystal structure of a human GABAA receptor
Miller, P. S., & Aricescu, A. R. (2014) · Nature · doi:10.1038/nature13293 · Cited by 710
Molecular evolution of vertebrate visual pigments
Yokoyama, S. (2000) · Progress in Retinal and Eye Research · doi:10.1016/s1350-9462(00)00002-1 · Cited by 586
Altenbach, C., Kusnetzow, A. K., Ernst, O. P., Hofmann, K. P., & Hubbell, W. L. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0802515105 · Cited by 462
Rhodopsin, photoreceptor of the rod cell. An emerging pattern for structure and function.
Khorana, H. G. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)48444-x · Cited by 434
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Metadata
- Authors
- Oprian, D. D., Molday, R. S., Kaufman, R. J., & Khorana, H. G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1987-12
- Volume
- 84
- Issue
- 24
- Pages
- 8874-8878
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2059441263
- Cited by
- 446
- References
- 1
- FWCI
- 3.72
- Type
- journal article
- Language
- en
- Open access
- green
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- Oprian, D. D., Molday, R. S., Kaufman, R. J., & Khorana, H. G. (1987). Expression of a synthetic bovine rhodopsin gene in monkey kidney cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.84.24.8874
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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