Cell adhesion induces expression of growth-associated genes in suspension-arrested fibroblasts.
Open accessAbstract
A methylcellulose suspension system that prevents cell-surface contact with the substrate was used to study the role of cell adhesion in the regulation of proliferation. The nonadhesive conditions established by suspension culture cause BALB/c 3T3 (A31) cells to enter a G0 state of growth arrest within 48 hr as defined by an inhibition of DNA synthesis and a suppression of c-myc and histone mRNA expression. The adhesion of these suspension-arrested cells rapidly induces c-fos, c-myc, and actin gene expression. This stimulation did not depend on the presence of serum since the adhesion of suspension-arrested cells, in the absence of serum, also induced the expression of c-fos and c-myc mRNAs. In addition, adhesion onto fibronectin increased the number of cells able to respond to epidermal growth factor and insulin and progress into S phase. These results indicate that adhesion of suspension-arrested cells activates the G0/G1 transition independent of growth factors.
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Cells embedded within a tissue interact with the extracellular matrix to develop cell type-dependent features such as morphology, function, proliferation, differentiation, gene expression pattern, and even survival.
“Cells embedded within a tissue interact with the extracellular matrix to develop cell type-dependent features such as morphology, function, proliferation, differentiation, gene expression pattern, and even survival (Dike and Farmer, 1988; Longhurst and Jennings, 1998; Damsky and Ilić, 2002).”
From In Vitro research Reproducibility: Keeping up High Standards (2019) · cites this paper as Dike and Farmer, 1988
The Results and Discussion (page numbers unavailable) show that extracellular-matrix adhesion affects cultured fibroblast morphology, growth and growth-related gene expression. The paper only suggests a role in differentiation and does not establish effects on survival or the full range of cell type-dependent features in tissues. (LLM, conf=0.87)
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Ignotz, R. A., & Massagué, J. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)35666-1 · Cited by 2,492 · see citing works
Benecke, B.-J., Ben-Ze'ev, A., & Penman, S. (1978) · Cell · doi:10.1016/0092-8674(78)90347-1 · Cited by 291 · see citing works
Kinetics of G1 transit following brief starvation for serum factors.
Campisi, J., Morreo, G., & Pardee, A. B. (1984) · Experimental Cell Research · doi:10.1016/0014-4827(84)90647-5 · Cited by 71 · see citing works
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Research works printed but not linked: 3
- [33]Elder, P. K., Schmidt, L. O., Ono, T. & Getz, M. J. (1984) Proc. Natl. Acad. Sci. USA 81, 7476–7480.
- [40]Farmer, S. R. (1986) in Cell and Molecular Biology of the Cytoskeleton, ed. Shay, J. W. (Plenum, New York), pp. 131–149.
- [47]Hirst, R., Horwitz, A., Buck, C. & Rohrschneider, L. (1986) Proc. Natl. Acad. Sci. USA 83, 6470–6474.
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In Vitro research Reproducibility: Keeping up High Standards
Hirsch, C., & Schildknecht, S. (2019) · Frontiers in Pharmacology · doi:10.3389/fphar.2019.01484 · Cited by 161 · see citing works
Monocyte, macrophage and foreign body giant cell interactions with molecularly engineered surfaces
Anderson, J. M., Defife, K., Mcnally, A., collier, T., & jenney, C. (1999) · Journal of Materials Science: Materials in Medicine · doi:10.1023/a:1008976531592 · Cited by 100 · see citing works
- Authors
- Dike, L. E., & Farmer, S. R.
- Affiliations
- Boston University (US)
- ORCID
- Farmer, S R
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1988-09
- Volume
- 85
- Issue
- 18
- Pages
- 6792-6796
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC282064
- OpenAlex
- W2024034599
- Cited by
- 171
- References
- 3
- Citations by year
- 2024: 2 · 2022: 2 · 2020: 3 · 2019: 2 · 2018: 3 · 2017: 1 · 2015: 2 · 2014: 2 · 2013: 1 · 2012: 1
- FWCI
- 3.19
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of General Medical Sciences (GM 29630)
- Topics
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Keywords
- cell adhesion, growth arrest, G0 to G1 transition, c-fos, c-Myc, actin gene expression, 3T3 fibroblasts, epidermal growth factor, insulin, fibronectin, cell proliferation regulation
- MeSH terms
- Actins, Animals, Cell Adhesion, DNA Replication, Fibroblasts, Fibronectins, Gene Expression Regulation, Growth Substances, Insulin, Mice, Inbred BALB C, Oncogenes, Transcription, Genetic, Mice
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