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Cited by 100Polymer Surface Interaction StudiesCell Adhesion Molecules ResearchCellular Mechanics and Interactions

Monocyte, macrophage and foreign body giant cell interactions with molecularly engineered surfaces

Anderson, J. M., Defife, K., Mcnally, A., collier, t., jenney, c.·1999·Journal of Materials Science: Materials in Medicine
DOI 10.1023/a:1008976531592
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Abstract

To elucidate the mechanisms involved in monocyte/macrophage adhesion and fusion to form foreign body giant cells on molecularly engineered surfaces, we have utilized our in vitro culture system to examine surface chemistry effects, cytoskeletal reorganization and adhesive structure development, and cell receptor-ligand interactions in in vitro foreign body giant cell formation. Utilizing silane-modified surfaces, monocyte/macrophage adhesion was essentially unaffected by surface chemistry, however the density of foreign body giant cells (FBGCs) was correlated with surface carbon content. An exception to the surface-independent macrophage adhesion were the alkyl-silane modified surfaces which exhibited reduced adhesion and FBGC formation. Utilizing confocal immunofluorescent techniques, cytoskeletal reorganization and adhesive structure development in in vitro FBGC formation was studied. Podosomes were identified as the adhesive structures in macrophages and FBGCs based on the presence of characteristic cytoplasmic proteins and F-actin at the ventral cell surface. Focal adhesion kinase (FAK) and focal adhesions were not identified as the adhesive structures in macrophages and FBGCs. In studying the effect of preadsorbed proteins on FBGC formation, fibronectin or vitronectin do not play major roles in initial monocyte/macrophage adhesion, whereas polystyrene surfaces modified with RGD exhibited significant FBGC formation. These studies identify the potential importance of surface chemistry-dependent conformational alterations which may occur in proteins adsorbed to surfaces and their potential involvement in receptor-ligand interactions. Significantly, preadsorption of alpha2-macroglobulin facilitated macrophage fusion and FBGC formation readily on the RGD surface in the absence of any additional serum proteins. As alpha2-macroglobulin receptors are not found on blood monocytes but are expressed only with macrophage development, these results point to a potential interaction between adsorbed 2-macroglobulin and its receptors on macrophages during macrophage development and fusion. These studies identify important surface independent and dependent effects in foreign body reaction development that may be important in the identification of biological design criteria for molecularly engineered surfaces and tissue engineered devices.

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  • As the foreign body response progresses, frustrated phagocytosis from activated macrophages leads to fusion into foreign body giant cells that attempt to further break down the implant.

    “As the FBR progresses, frustrated phagocytosis from activated macrophages will lead to the fusion of macrophages into foreign body giant cells (FBGCs) that attempt to further breakdown the implant.73,94,97–98”

    From Biocompatible Materials for Continuous Glucose Monitoring Devices (2013) · cites this paper as [97]

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Authors
Anderson, J. M., Defife, K., Mcnally, A., collier, T., & jenney, C.
Affiliations
Case Western Reserve University (US)
ORCID
Anderson, J. M.
Venue
Journal of Materials Science: Materials in Medicine
Publisher
Springer Science and Business Media LLC
Published
1999-10
Volume
10
Issue
10-11
Pages
579-588
ISSN
  • 0957-4530 (ISSN-L)
  • 1573-4838
DOI
10.1023/a:1008976531592
OpenAlex
W1211886664
Cited by
100
References
34
Citations by year
2026: 2 · 2025: 1 · 2024: 5 · 2023: 3 · 2022: 2 · 2021: 6 · 2019: 4 · 2018: 2 · 2017: 5 · 2016: 1 · 2015: 6 · 2014: 4 · 2013: 4 · 2012: 9
FWCI
2.57
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Journal of Materials Science Materials in Medicine (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health
Topics
  • Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
foreign body giant cells, foreign body reaction, macrophage adhesion, surface carbon content, cytoskeletal remodeling, podosomes, F-actin, focal adhesions, focal adhesion kinase, RGD peptide, alpha2-macroglobulin, fibronectin, vitronectin, macrophage development

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